RT Journal Article
T1 The Subgingival Microbiome in Patients with Down Syndrome and Periodontitis
A1 Nóvoa Garrido, Lourdes
A1 Sánchez, María del Carmen
A1 Blanco Carrión, Juan
A1 Limeres Posse, Jacobo
A1 Cuenca, Maigualida
A1 Marín, María José
A1 Sanz, Mariano
A1 Herrera, David
A1 Diz Dios, Pedro
K1 Down syndrome
K1 Periodontitis
K1 Microbiome
K1 Microbial community
K1 DNA sequencing
AB Objective: To describe the subgingival microbiome of individuals with Down syndrome (DS). Methods: We conducted a cross-sectional observational study that obtained bacterial DNA samples from 50 patients with DS, 25 with periodontitis (PDS) and 25 with a healthy periodontal condition (HDS). The samples were analyzed by sequencing the 16S rRNA gene V3–V4 hypervariable region using the MiSeq System. Taxonomic affiliations were assigned using the naïve Bayesian classifier integrated in QIIME2 plugins. We evaluated the difference in bacteria abundance between the sample groups using Wilcoxon and Kruskal–Wallis tests. We evaluated the alpha diversity of the identified species using the Observed, Chao1metric, ACE and Shannon indices and evaluated beta diversity with principal coordinate analysis (registration code: 2018/510). Results: Twenty-one genera and 39 bacterial species showed a significantly different abundance between the study groups. Among the genera, Porphyromonas, Treponema, Tannerella and Aggregatibacter were more abundant in the PDS group than in the HDS group, as were the less commonly studied Filifactor, Fretibacterium and Desulfobulbus genera. Among the species, Porphyromonas spp. and Tannerella spp. were the most abundant in the PDS group; the most abundant species in the HDS group were Pseudomonas spp., Granulicatella spp. and Gemella spp. Conclusion: Well-recognized periodontal pathogens and newly proposed pathogenic taxa were associated with periodontitis in patients with DS
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/23871
UL http://hdl.handle.net/10347/23871
LA eng
NO Nóvoa, L.; Sánchez, M.C.; Blanco, J.; Limeres, J.; Cuenca, M.; Marín, M.J.; Sanz, M.; Herrera, D.; Diz, P. The Subgingival Microbiome in Patients with Down Syndrome and Periodontitis. J. Clin. Med. 2020, 9, 2482
NO This project was co-funded by Xunta de Galicia under Ignicia Programme, Axencia Galega de Innovación, GAIN (12/08/2016; GRANT_NUMBER: IN855A)
DS Minerva
RD 28 abr 2026