RT Journal Article
T1 Identification and Molecular Characterization of Superoxide Dismutases Isolated From A Scuticociliate Parasite: Physiological Role in Oxidative Stress
A1 Folgueira López, Iria
A1 Lamas Fernández, Jesús
A1 Felipe González, Ana Paula de
A1 Sueiro Benavides, Rosa Ana
A1 Leiro Vidal, José Manuel
K1 Scuticociliate Parasite
K1 Philasterides dicentrarchi
K1 Parasitic disease
K1 Farmed fish
K1 Oxidative stress
AB Philasterides dicentrarchi is a free-living microaerophilic scuticociliate that can become a facultative parasite and cause a serious parasitic disease in farmed fsh. Both the free-living and parasitic forms of this scuticociliate are exposed to oxidative stress associated with environmental factors and the host immune system. The reactive oxygen species (ROS) generated by the host are neutralized by the ciliate by means of antioxidant defences. In this study we aimed to identify metalloenzymes with superoxide dismutase (SOD) activity apable of inactivating the superoxide anion (•O2−) generated during induction of oxidative stress. P. dicentrarchi possesses the three characteristic types of SOD isoenzymes in eukaryotes: copper/zinc-SOD, manganese-SOD and iron-SOD. The Cu/Zn-SOD isoenzymes comprisethree types of homodimeric proteins (CSD1-3) of molecular weight (MW) 34–44kDa and with very diferent AA sequences. All Cu/Zn-SODs are sensitive to NaCN, located in the cytosol and in the alveolar sacs, and one of them (CSD2) is extracellular. Mn- and Fe-SOD transcripts encode homodimeric proteins (MSD and FSD, respectively) in their native state: a) MSD (MW 50kDa) is insensitive to H2O2 and NaN3 and is located in the mitochondria; and b) FSD (MW 60kDa) is sensitive to H2O2, NaN3 and the polyphenol trans-resveratrol and is located extracellularly. Expression of SOD isoenzymes increases when •O2 − is induced by ultraviolet (UV) irradiation, and the increase is proportional to the dose of energy applied, indicating that these enzymes are actively involved in cellular protection against oxidative stress
PB Nature Publishing
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21027
UL http://hdl.handle.net/10347/21027
LA eng
NO Folgueira, I., Lamas, J., de Felipe, A.P. et al. Identification and Molecular Characterization of Superoxide Dismutases Isolated From A Scuticociliate Parasite: Physiological Role in Oxidative Stress. Sci Rep 9, 13329 (2019). https://doi.org/10.1038/s41598-019-49750-5
NO This study was financially supported by grant AGL2017-83577-R awarded by the Ministerio de Economíay Competitividad (Spain) and Fondo Europeo de Desarrollo Regional -FEDER- (European Union), by grantED431C2017/31 from the Xunta de Galicia (Spain), and by PARAFISHCONTROL project, which receivedfunding from the European Union’s Horizon 2020 research and innovation programme under grant agreementNo. 634429
DS Minerva
RD 29 abr 2026