RT Journal Article T1 Determination of exposure to Fasciola hepatica in horses from Uruguay using a recombinant-based ELISA A1 Sanchís, J. A1 Suárez, J. A1 Hillyer, G.V. A1 Hernández Malagón, José Ángel A1 Solari, M.A A1 Cazapal Monteiro, Cristiana Filipa A1 Duque de Araujo, A.M. A1 Carvalho, Luís Manuel Madeira de A1 Paz Silva, Adolfo A1 Sánchez-Andrade Fernández, Rita A1 Arias Vázquez, María Sol K1 Fasciola hepatica K1 Equine K1 Uruguay K1 Sensitisation K1 FhrAPS AB The risk of exposure to Fasciola hepatica in horses from Uruguay was evaluated using ELISA and a recombinant surface protein (FhrAPS). Blood samples were collected from 368 horses from different districts. Detection of antibodies and the seasonal IgG pattern against the trematode was also established. The overall seroprevalence was 54% (ranging 23% November to 93% December). Significantly higher values were observed in the Anglo-Arab horses (86%) and in those older than xix year (63%). No sex- or origin-differences were observed. Two primary risk periods for infection in Uruguay have been identified, the end of spring and autumn. Exposure to the fluke should be taken into account among those horses feeding on pastures grazed by ruminants. Using FhrAPS-ELISA, helpful information concerning the risk of horses to the infection by trematode can be obtained. This test allows the opportunity to compare the results achieved in different laboratories by minimising specific regional effects. PB CZECH ACADEMY AGRICULTURAL SCIENCES SN 0375-8427 YR 2015 FD 2015-09-30 LK https://hdl.handle.net/10347/38910 UL https://hdl.handle.net/10347/38910 LA eng NO Sanchis J, Suarez J, Hillyer GV, Hernandez JA, Solari MA, Cazapal-Monteiro C, et al.. Determination of exposure to Fasciola hepatica in horses from Uruguay using a recombinant-based ELISA. Vet Med-Czech. 2015;60(9):483-488. doi: 10.17221/8439-VETMED. NO Partially supported by the Xunta de Galicia, Spain (Research Project No. 10MDS261023PR) and Ministerio de Economia y Competitividad, Spain; FEDER (Research Project No. AGL2012-34355), and complies with the current laws for Animal Health Research in Spain. DS Minerva RD 23 abr 2026