RT Journal Article
T1 Integrity and quantity of salivary cell-free DNA as a potential molecular biomarker in oral cancer: a preliminary study
A1 Rapado González, Óscar
A1 López Cedrún, José Luis
A1 Lago Lestón, Ramón
A1 Abalo Piñeiro, Alicia
A1 Rubin Roger, Guillermo
A1 Salgado Barreira, Ángel
A1 López López, Rafael
A1 Muinelo Romay, Laura
A1 Suárez Cunqueiro, María Mercedes
K1 ALU repeat
K1 DNA integrity
K1 Liquid biopsy
K1 Oral Squamous Cell Carcinoma (OSCC)
K1 Saliva
K1 Salivary cell-free DNA (scfDNA)
PB Wiley
SN 0904-2512
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29238
UL http://hdl.handle.net/10347/29238
LA eng
NO J Oral Pathol Med.2022;51:429–435
NO Background: differences in cell-free DNA (cfDNA) fragments have been described as a valuable tool to distinguish cancer patients from healthy individuals. We aim to investigate the concentration and integrity of cfDNA fragments in saliva from oral squamous cell carcinoma (OSCC) patients and healthy individuals in order to explore their value as diagnostic biomarkers. Methods: saliva samples were collected from a total of 34 subjects (19 OSCC patients and 15 healthy controls). The total concentration of salivary cfDNA (scfDNA) was determined using a fluorometry method and quantitative real-time polymerase chain reaction (qPCR). To evaluate the scfDNA quantity and integrity, qPCR targeting Arthobacter luteus (ALU) sequences at three amplicons of different lengths (60, 115, and 247 bp, respectively) was carried out. ScfDNA integrity indexes (ALU115/ALU60 and ALU247/ALU60) were calculated as the ratio between the absolute concentration of the longer amplicons 115 bp and 247 bp and the total scfDNA amount (amplicon 60 bp).Results: the total scfDNA concentration (ALU60) was higher in OSCC than in healthy donors, but this trend was not statistically significant. The medians of scfDNA integrity indexes, ALU115/ALU60 and ALU247/ALU60, were significantly higher in OSCC, showing area under the curve values of 0.8211 and 0.7018, respectively. Conclusion: our preliminary results suggest that scfDNA integrity indexes (ALU115/ALU60 and ALU247/ALU60) have potential as noninvasive diagnostic biomarkers for OSCC
DS Minerva
RD 28 abr 2026