RT Journal Article
T1 Parentage assignment of progeny in mixed milt fertilization of Caspian brown trout Salmo trutta caspius using microsatellite DNA markers: Implications for conservation
A1 Sourinejad, Iman
A1 Kalbassi, Mohammad Reza
A1 Pino Querido-Ferreira, Ania Augusta
A1 Vera Rodríguez, Manuel
A1 Bouza Fernández, María Carmen
A1 Martínez Portela, Paulino
K1 Parentage assignment
K1 Effective population size
K1 Genetic diversity
K1 Salmo trutta caspius
AB Parentage of a stock of mixed milt produced progeny in current artificial breeding protocol of endangered Caspian brown trout, Salmo trutta caspius, was determined using three microsatellite loci chosen after a primary analysis of genetic diversity at nine microsatellite loci in the eight used breeder individuals. Overall, 98.8% of progeny were assigned to their parents using Family Assignment Program (FAP). Selection of hyper-variable microsatellites in Caspian brown trout to identify unique alleles was effective for unambiguous parentage determination and estimation of genetic diversity in this study. Effective population size of breeder individuals (Ne) was lower than the number of breeder individuals used (Nb) indicating unbalanced contribution of breeder individuals to progeny. Indeed, one of the four male breeder individuals produced about 70 % and the other three produced only from 4.86 % to 18.83 % of progeny. The average observed and expected heterozygosity of progeny (0.723 ± 0.011 and 0.684 ± 0.009, respectively) was significantly lower than that of their parents (0.833 and 0.800, respectively). Our data indicate that the current breeding protocol of Caspian brown trout may not provide equal opportunity for all the breeder individuals to contribute equally to progeny. Therefore, appropriate fertilization designs in the hatchery should be established in order to equalize the genetic contribution of different breeder individuals
PB African Journals OnLine (AJOL)
SN 1684–5315
YR 2011
FD 2011
LK http://hdl.handle.net/10347/26148
UL http://hdl.handle.net/10347/26148
LA eng
NO African Journal of Biotechnology Vol. 10(26), pp. 5084-5090. DOI: 10.5897/AJB10.664
DS Minerva
RD 30 abr 2026