RT Journal Article
T1 Sulfamethoxazole enhances specific enzymatic activities under aerobic heterotrophic conditions: a metaproteomic approach
A1 Kennes Veiga, David Manuel
A1 Trueba Santiso, Alba María
A1 Gallardo Garay, Valentina
A1 Balboa Méndez, Sabela
A1 Carballa Arcos, Marta
A1 Lema Rodicio, Juan Manuel
K1 Biotransformation
K1 Genetics
K1 Organic compounds
K1 Peptides and proteins
K1 Sludges
AB The growing concern about antibiotic-resistant microorganisms has focused on the sludge from wastewater treatment plants (WWTPs) as a potential hotspot for their development and spread. To this end, it seems relevant to analyze the changes on the microbiota as a consequence of the antibiotics that wastewater may contain. This study aims at determining whether the presence of sulfamethoxazole (SMX), even in relatively low concentrations, modifies the microbial activities and the enzymatic expression of an activated sludge under aerobic heterotrophic conditions. For that purpose, we applied a metaproteomic approach in combination with genomic and transformation product analyses. SMX was biotransformed, and the metabolite 2,4(1H,3H)-pteridinedione-SMX (PtO-SMX) from the pterin-conjugation pathway was detected at all concentrations tested. Metaproteomics showed that SMX at 50–2000 μg/L slightly affected the microbial community structure, which was confirmed by DNA metabarcoding. Interestingly, an enhanced activity of the genus Corynebacterium and specifically of five enzymes involved in its central carbon metabolism was found at increased SMX concentrations. Our results suggest a role of Corynebacterium genus on SMX risks mitigation in our bioreactors
PB ACS Publications
SN 0013-936X
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29673
UL http://hdl.handle.net/10347/29673
LA eng
NO Environ. Sci. Technol. 2022, 56, 18, 13152–13159
NO This research was funded by the Spanish Government (Agencia Estatal de Investigación) through the ANTARES project (PID2019-110346RB-C21), a PhD Xunta de Galicia Grant (ED481A-2018/113, D.M.K.-V.) and a Juan de la Cierva-Formación postdoctoral grant (FJC2019-041664-I, A.T.-S.). The authors belong to the Galician Competitive Research Groups (GRC_ ED431C-2021/37)
DS Minerva
RD 24 abr 2026