RT Journal Article
T1 Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis
A1 Novo, Mercedes
A1 Illodo Brea, Sara
A1 Seijas Cerceda, Jesús
A1 Moraes, Stella Hernández Faria De
A1 Rodríguez-Prieto, Flor
A1 Al-Soufi, Wajih
K1 Alzheimer’s disease
K1 Soluble amyloid-b (Ab) oligomers
K1 Pathogenesis
K1 diagnosis
AB Alzheimer's disease (AD) is a major public health challenge, with its onset occurring years before symptoms appear. Soluble amyloid-β (Aβ) oligomers are key species in AD pathogenesis and diagnosis, highlighting the need for early detection. This study investigates the intrinsic fluorescence of Aβ(1–40) (Aβ40) as a label-free approach to detecting early-stage oligomers. Aβ40 exhibits autofluorescence dominated by tyrosine emission, which undergoes a strong blue shift and quenching during oligomerization. Additionally, aggregation-induced emission (AIE) in the visible spectral region emerges, correlating with Aβ oligomer concentration and providing a means to detect and quantify oligomers. At the critical aggregation concentrations cac1 = 0.5 μM and cac2 = 19 μM, distinct aggregation behaviours are observed. By employing steady-state fluorescence spectroscopy, a widely accessible technique, these findings establish a direct link between early Aβ aggregation and intrinsic fluorescence changes. This approach eliminates the need for extrinsic probes, simplifying experimental procedures and reducing artefacts. Although further studies are required to develop a robust quantitative correlation for potential diagnostic applications, Aβ autofluorescence represents a promising strategy for investigating early aggregation processes in the context of AD
PB Royal society of chemistry
SN 1463-9084
YR 2025
FD 2025-07-21
LK https://hdl.handle.net/10347/43532
UL https://hdl.handle.net/10347/43532
LA eng
NO Novo, M., Illodo, S., Seijas, J., & Rodríguez, J. (2025). Intrinsic visible emission of amyloid-β oligomers: a potential tool for early Alzheimer’s diagnosis. Physical Chemistry Chemical Physics,(27), 29067-29078. https://doi.org/10.1039/D5CP01547B
NO We thank the Spanish Ministerio de Ciencia e Innovación for their financial support (PID2020-120378RB-I00). S. I. and S. H. thank the Xunta de Galicia for their research scholarships (ED481A2021/211, ED481A-2024-083). All authors contributed equally to this work.
DS Minerva
RD 28 abr 2026