RT Journal Article
T1 Profiling of Sub-Lethal in Vitro Effects of Multi-Walled Carbon Nanotubes Reveals Changes in Chemokines and Chemokine Receptors
A1 Keshavan, Sandeep
A1 Torres Andón, Fernando
A1 Gallud, Audrey
A1 Chen, Wei
A1 Reinert, Knut
A1 Tran, Lang
A1 Fadeel, Bengt
K1 Multi-walled carbon nanotubes
K1 Nanoparticles
K1 Chemokines
K1 Macrophages
K1 Transcriptomics
AB Engineered nanomaterials are potentially very useful for a variety of applications, but studies are needed to ascertain whether these materials pose a risk to human health. Here, we studied three benchmark nanomaterials (Ag nanoparticles, TiO2 nanoparticles, and multi-walled carbon nanotubes, MWCNTs) procured from the nanomaterial repository at the Joint Research Centre of the European Commission. Having established a sub-lethal concentration of these materials using two human cell lines representative of the immune system and the lungs, respectively, we performed RNA sequencing of the macrophage-like cell line after exposure for 6, 12, and 24 h. Downstream analysis of the transcriptomics data revealed significant effects on chemokine signaling pathways. CCR2 was identified as the most significantly upregulated gene in MWCNT-exposed cells. Using multiplex assays to evaluate cytokine and chemokine secretion, we could show significant effects of MWCNTs on several chemokines, including CCL2, a ligand of CCR2. The results demonstrate the importance of evaluating sub-lethal concentrations of nanomaterials in relevant target cells
PB MDPI
YR 2021
FD 2021
LK http://hdl.handle.net/10347/25286
UL http://hdl.handle.net/10347/25286
LA eng
NO Nanomaterials 2021, 11(4), 883; https://doi.org/10.3390/nano11040883
NO This research was funded by the European Commission through the FP7 project MARINA (grant agreement no. 263215) and the Horizon 2020 project BIORIMA (grant agreement no. 760928)
DS Minerva
RD 28 abr 2026