RT Book,_Section T1 Modification of the method to determine dehydrogenase activity in soils spiked with 1-butyl-3-methylimidazolium tetrafluoroborate (BMIMBF4) A1 Bello Currás, Diana A1 Muiño Blanco, Fátima A1 García Carballal, Sara A1 Salgado Carballo, Josefa A1 Trasar Cepeda, María del Carmen K1 Tetrafluoroborate 1-butyl-3-methylimidazolium (BMIMBF4) K1 Dehydrogenase activity K1 Soil contamination K1 Soil enzymes AB The ionic liquids (ILs) are often denominated as “green fluids”, because they are considered as innocuous due to their negligible volatility. Due to this apparent innocuousness, the ILs are attracted a great attention as an alternative to traditional organic solvents, highly contaminant. However, the problem of contamination is not limited to the atmosphere, since also aquatic and terrestrial environments can be affected. Therefore, and because the toxic effects of ILs on the environment are largely unknown, research studies are needed to deepen this aspect before to assign them the appellation of “green fluids”. Nevertheless, and despite the increased number in the last few years of studies on different aspects of ILs, the toxicity of these compounds to the environment in general, and to the soils in particular, only rarely is investigated. One of the most used ILs in different applications is 1-butyl-3-methylimidazolium tetrafluoroborate (BMIMBF4). Among all the soil properties, those determining the edaphic metabolism, and especially the enzymatic activities, are highly sensitive to ascertain the disturbance caused by any external agent. Given the lack of studies investigating the effect of ILs on the edaphic environment, before the use of enzymatic activities as indicators of the toxicity for soils of any given IL, it is necessary to test if classical methods to determine the soil enzymatic activities are adequate for their use in soils spiked with that given IL.In this study we investigated the suitability of dehydrogenase method to determine the activity of this enzyme in soils with spiked BMIMBF4, modifying the method according to the problems identified PB MDPI SN 978-3-03842-192-4 YR 2016 FD 2016 LK http://hdl.handle.net/10347/25737 UL http://hdl.handle.net/10347/25737 LA eng NO Bello, D., Muiño, F., García-Carballal, S., Salgado, J. & Trasar-Cepeda, C. (2016). Modification of the method to determine dehydrogenase activity in soils spiked with 1-butyl-3-methylimidazolium tetrafluoroborate (BMIMBF4). In J.A. Seijas, M.P. Vázquez Tato y S.K. Lin. (Ed.), Proceedings ECSOC-20: The 20Th International Electronic Conference On Synthetic Organic Chemistry: November 1-30, 2016. MDPI. doi: 10.3390/ecsoc-20-f001 NO The 20th International Electronic Conference on Synthetic Organic Chemistry session Ionic Liquids NO his research was financed by the Spanish Ministerio de Economía y Competitividad (Project No. CGL2015-66857-C2-1-R) DS Minerva RD 22 abr 2026