RT Journal Article
T1 Cationic photosensitizers and potassium iodide: an innovative approach for enhanced photodynamic inactivation of pathogenic bacteria in aquaculture
A1 Vieira, Cátia
A1 Bartolomeu, Maria
A1 Monteiro, Carlos Jorge Pereira
A1 López Romalde, Jesús
A1 Gallego Veigas, Pedro Pablo
A1 Neves, Maria da Graça de Pinho Morgado Silva
A1 Faustino, Maria do Amparo Ferreira
A1 Almeida, Maria Adelaide de Pinho
K1 Farmed fish
K1 Bacterial infections
K1 Antimicrobial
K1 Charged porphyrins
K1 Methylene blue
K1 Potassium iodide
K1 Vibrio spp.
K1 Escherichia coli
AB Aquaculture is a rapidly growing industry, accounting for more than half of the worldwide fish production. However, this sector is often affected by bacterial infections, threatening farmed animals and consumer safety. This study explored the potential of Photodynamic Inactivation (PDI) as an innovative antimicrobial approach for the inactivation of Gram-negative bacteria relevant to the aquaculture sector: Vibrio parahaemolyticus, Vibrio anguillarum, and Escherichia coli. The experiments were conducted in the presence of the cationic photosensitizers (PSs) 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin (TMPyP) and methylene blue (MB). For V. parahaemolyticus, the protocol was extended to the neutral and anionic PSs curcumin (CUR), chlorin e6 trimethyl ester (Ce6Me), and 5,10,15,20-tetrakis(4-sulfophenyl)porphyrin (TPPS4). The assays were performed in the absence or presence of potassium iodide, well-known to improve PDI effect, while its potential for aquaculture application is yet to be studied. These evaluations were performed in phosphate-buffered saline (PBS) and in artificial seawater (ASW) to simulate aquaculture water disinfection. The cationic PSs TMPyP and MB were the most efficient in the bacterial inactivation. Although higher concentrations of PS were required to achieve effective bacterial inactivation in ASW compared to PBS, the application of KI enhanced the effectiveness of all PSs, reducing the treatment time at least by threefold. The bacterial inactivation profiles revealed higher susceptibility of Vibrionaceae bacteria which may be linked to the differential PDI impact on the outer cell membranes of these bacteria. These findings indicate that treatments with PSs, particularly TMPyP and MB, in combination with KI constitute a potential approach for bacteria control in the aquaculture sector.
PB Elsevier
SN 0044-8486
YR 2024
FD 2024-11-13
LK https://hdl.handle.net/10347/42377
UL https://hdl.handle.net/10347/42377
LA eng
NO Cátia Vieira, Maria Bartolomeu, Carlos J.P. Monteiro, Jesús L. Romalde, Pedro P. Gallego, M. Graça P.M.S. Neves, M. Amparo F. Faustino, Adelaide Almeida, Cationic photosensitizers and potassium iodide: an innovative approach for enhanced photodynamic inactivation of pathogenic bacteria in aquaculture, Aquaculture, Volume 596, Part 2, 2025, 741882, ISSN 0044-8486, https://doi.org/10.1016/j.aquaculture.2024.741882
NO This research was support by the University of Aveiro and FCT/MCTES (Fundação para a Ciência e a Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through CESAM (UID/AMB/50017/2019 and UIDB/50017/2020 + UIDP/50017/2020) and LAQV-REQUIMTE (LA/P/0008/2020 DOI 10.54499/LA/P/0008/2020, UIDP/50006/2020 DOI 10.54499/UIDP/50006/2020 and UIDB/50006/2020 DOI 10.54499/UIDB/50006/2020), research units and to project PREVINE - FCT-PTDC/ASP-PES/29576/2017), through national funds (OE) and where applicable co-financed by the FEDER-Operational Thematic Program for Competitiveness and Internationalization-COMPETE 2020, within the PT2020 Partnership Agreement funded by national funds.
DS Minerva
RD 29 abr 2026