RT Journal Article
T1 Oral specimens as a tool for accurate metagenomic analysis: A pilot study
A1 Rodriguez-Fuentes, Manuel Eros
A1 Pérez-Sayáns García, Mario
A1 Barbeito Castiñeiras, Gema
A1 Molares-Vila, Alberto
A1 Prado-Pena, Irene B.
A1 Vianna Camolesi, Gisela Cristina
A1 López López, Rafael
K1 Metagenomics
K1 Microbiota
K1 Mouth
K1 Mouth mucosa
K1 Saliva
AB ObjectivesAcute oral mucosal damage, as well as other inflammatory processes seem to be related to dysbiosis of the oral microbiome. The need to study changes in the oral microbiome led us to hypothesize what type of sample would provide the most representative picture of the entire human oral microbiome.Materials and methodsAn observational, and cross-sectional study was carried out. Six healthy adult participants provided 3 different sample types each, that included saliva, oral rinse and mucosal biopsy tissue. We performed 16S rRNA sequencing of the V3-V4 region of the 18 samples using Illumina MiSeq technology.ResultsParticipants were 27 ± 6,3 years old. Bacterial alpha diversity was higher in oral rinse samples compared to whole unstimulated saliva and oral mucosa tissue (p = 0,005). However, saliva specimens showed a 56 % relative abundance of identified species followed by a 30 % in oral rinse and only 1 % in tissue samples.ConclusionsThis study found differences on oral microbiome composition for each type of sample. Oral rinse should be chosen when higher alpha diversity is needed, whereas whole unstimulated saliva should be more appropriate for larger amount of bacterial DNA.Clinical relevanceThe results obtained demonstrate the importance of a correct choice of the optimal type of oral sample for microbiome studies due to the differences found in its composition.
PB Elsevier
SN 2468-8509
YR 2024
FD 2024-07-30
LK https://hdl.handle.net/10347/39752
UL https://hdl.handle.net/10347/39752
LA eng
NO References  Rodríguez-Fuentes, M. E., Pérez-Sayáns, M., Barbeito-Castiñeiras, G., Molares-Vila, A., Prado-Pena, I. B., Camolesi, G. C. V., & López-López, R. (2024). Oral specimens as a tool for accurate metagenomic analysis: A pilot study. Journal of Stomatology, Oral and Maxillofacial Surgery, 125(5, Supplement 2)10.1016/j.jormas.2024.101991
NO Molares-Vila. A. is supported by a fellowship co-funded by the State Investigation Agency from the Spanish Ministry of Science and Innovation (MCIN/AEI/10.13039/501100011033) through the procedure file “PTA2021-019927-I” and by the European Social Fund+. No other funds, grants or support was received for conducting this study.
DS Minerva
RD 5 may 2026