RT Journal Article
T1 Fast HPLC-MS/MS method for determining penicillin antibiotics in infant formulas using molecularly imprinted solid-phase extraction
A1 Díaz Bao, Mónica
A1 Barreiro Tomé, Rocío
A1 Miranda López, José Manuel
A1 Cepeda Sáez, Alberto
A1 Regal López, Patricia
K1 Antibiotics
K1 Infant formulas
K1 Penicillin
K1 Solid-Phase extraction
AB The dairy cattle may suffer from different infections relatively often, but the inflammation of the mammary gland is very important to the farmer. These infections are frequently treated with penicillin antimicrobial drugs. However, their use may result in the presence of residues in animal products, such as milk powder and/or infant formulas, and it represents a potential risk for consumers. To monitor this, the EU has defined safe maximum residue limits (MRLs) through Commission Regulation (EU) number 37/2010. Although LC-MS is a trustful option for confirmation and quantification of antibiotics, the analysis of real samples with complex matrices frequently implies previous clean-up steps. In this work, precipitation polymerization has been used and different molecularly imprinted polymer (MIP) sorbents were tested and optimized for the fast and simultaneous solid-phase extraction (MISPE) of eight common penicillins (ampicillin, amoxicillin, oxacillin, penicillin G, penicillin V, cloxacillin, dicloxacillin, and nafcillin). The extracts were analyzed using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and the applicability of these polymers as sorbents for the extraction of penicillins at MRL levels in milk powder (infant formulas) was proved. The limits of detection and quantification were below the legal tolerances, except for LOQ for oxacillin and cloxacillin
PB Hindawi
SN 2090-8865
YR 2015
FD 2015
LK http://hdl.handle.net/10347/21654
UL http://hdl.handle.net/10347/21654
LA eng
NO Díaz-Bao, M., Barreiro, R., Miranda, J. M., Cepeda, A., & Regal, P. (2015). Fast HPLC-MS/MS method for determining penicillin antibiotics in infant formulas using molecularly imprinted solid-phase extraction. Journal of Analytical Methods in Chemistry, 2015, 959675
NO This research was supported by the Project EM 2012/153 from Consellería de Cultura, Educación e Ordenacion Universitaria, Xunta de Galicia
DS Minerva
RD 24 abr 2026