RT Journal Article
T1 Comparative analysis of multidrug-resistant Klebsiella pneumoniae strains of food and human origin reveals overlapping populations
A1 Silva Bea, Sergio
A1 Romero Bernárdez, Manuel
A1 Parga Martínez, Ana
A1 Fernández, Javier
A1 Mora, Azucena
A1 Otero Casal, Ana María
K1 ESBL
K1 Zoonotic
K1 Biofilm
K1 Serum resistance
K1 Hypermucoviscosity
AB Given the increasing incidence of multidrug-resistant (MDR) Klebsiella pneumoniae infections, it is of great interest to investigate the risk of transmission associated with the prevalence of this pathogen. Some studies have described fresh raw poultry meat as a reservoir of MDR K. pneumoniae, including clinically relevant sequence types (ST) and extended-spectrum β-lactamase (ESBL) strains, indicating possible consumer exposure. This study compared 47 MDR strains of K. pneumoniae from poultry meat and human clinical isolates to assess similarities, including analysis of antimicrobial resistance profiles and virulence factors involved in infection. In addition, several biofilm culture methods were evaluated for reproducible assessment of biofilm formation in K. pneumoniae strains. Globally, no association between strain origin and STs, hypermucoviscosity, biofilm formation or serum resistance could be found between isolates of food and clinical origin, nor an associated AMR pattern, suggesting overlapping populations. We found that LB supplemented with glucose in microaerobiosis was the best discrimination condition for biofilm formation in the active attachment biofilm cultivation model. The biofilm formation capacity was strongly dependent on culture conditions, with a strain-specific response, but only a minor increase in biofilm levels was recorded in clinical K. pneumoniae populations. Our results suggest that a similar risk of zoonosis transmission from potentially virulent foodborne strains previously observed in E. coli is also present in this high-priority pathogen. This study further confirms that foodborne isolates of K. pneumoniae pose a risk to consumers and therefore this pathogen should be included in the surveillance of foodborne pathogens with high risk of MDR infections and therapeutic failure
PB Elsevier
SN 0168-1605
YR 2024
FD 2024
LK http://hdl.handle.net/10347/33801
UL http://hdl.handle.net/10347/33801
LA eng
NO International Journal of Food Microbiology, Volume 413, 2024, 110605
NO The experiments performed in this work were financed with the projects and funds PID2019-104439RB-C21/AEI/10.13039/501100011033 (Agencia Estatal de Investigación, Spain); ED431B 2020/13 and ED431C 2021/11 (Xunta de Galicia); ED431E 2018/09 (BioReDes); GRUPIN IDI/2022/000033 by the Regional Ministry of Science of Asturias (IDI/2022/000033). Sergio Silva-Bea was supported with the “Formación de Profesorado Universitario” program (FPU21/01147). Manuel Romero was supported by the Maria Zambrano program from the Spanish Ministry of Universities
DS Minerva
RD 23 abr 2026