RT Journal Article
T1 An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro
A1 Fuentes Iglesias, Alejandro
A1 García Outeiral, Vera
A1 Pardavila Paz, José Ángel
A1 Wang, Jianlong
A1 Fidalgo Pérez, Miguel Ángel
A1 Guallar Artal, Diana
K1 Immunoprecipitation Protocol
K1 Protein-RNA Interactions
K1 RNA-binding proteins
K1 in vitro RNA immunoprecipitation
K1 iv-RIP
AB RNA-binding proteins are key regulators of cell identity and function, which underscores the need for unbiased and versatile protocols to identify and characterize novel protein-RNA interactions. Here, we describe a simple and cost-effective in vitro RNA immunoprecipitation (iv-RIP) method to assess the direct or indirect RNA-binding ability of any protein of interest. The versatility of this method relies on the adaptability of the immunoprecipitation conditions and the choice of the RNA, which exponentially broadens the range of potential applications
PB Cell Press
YR 2020
FD 2020-09-04
LK https://hdl.handle.net/10347/38657
UL https://hdl.handle.net/10347/38657
LA eng
NO Fuentes-Iglesias et al., An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro, STAR Protocols (2020), https://doi.org/10.1016/j.xpro.2020.100093
DS Minerva
RD 29 abr 2026