RT Journal Article
T1 Monitoring of the shedding and serological dynamics of Bovine gammaherpesvirus type 4 in a dairy cattle herd
A1 Díaz Cao, José Manuel
A1 Prieto Lago, Alberto
A1 López-Lorenzo, Gonzalo
A1 López Novo, Cynthia
A1 Iglesias Becerra, Antonio
A1 Díaz Fernández, Pablo
A1 Moral, Javier
A1 López Sández, Ceferino Manuel
A1 Díez Baños, Pablo
A1 Morrondo Pelayo, María Patrocinio
A1 Fernández Rodríguez, Gonzalo
A1 Panadero Fontán, Rosario
K1 BoHV-4
K1 Cattle
K1 Heifers
K1 PCR
K1 Serology
AB Bovine gammaherpesvirus type 4 (BoHV-4) is increasingly related with reproductive disease in cattle, but its epidemiology is not fully understood. We monitored the serological response and shedding of BoHV-4 in a positive dairy cattle farm with metritis. First, we performed an ELISA to detect BoHV-4 antibodies in all the animals (n = 104). Afterwards, ten seronegative heifers introduced in the production lot and sera samples were monthly taken for four months and then 6-10 months after introduction to detect BoHV-4 antibodies by ELISA. Moreover, a vaginal swab was taken after calving to detect BoHV-4 by PCR. Concurrently, a weekly collection of vaginal and nasal swabs and milk was performed during the first month post-partum in multiparous cows with metritis (n = 14), heifers with metritis (n = 4), heifers without metritis but positive to BoHV-4 (ELISA or PCR) (n = 2) and multiparous cows without metritis (n = 3). Seropositivity was higher in older animals and in the production lot. Three heifers which shed BoHV-4 after parturition resulted seronegative at first but eventually seroconverted. In the same vein, most heifers seroconverted after 6-10 months in the production lot (8/10). Multiparous cows shed virus by various routes: 13/14 (93 %) in vaginal secretions, 7/14 (50 %) in nasal exudates and 7/14 (50 %) in milk. However, in the other groups, shedding was only detected in vaginal swabs from the first week post-partum. Our study describes BoHV-4 shedding in field conditions. Seronegative animals may become horizontally infected when moved to a contaminated environment
PB Elsevier
SN 0378-1135
YR 2019
FD 2019
LK http://hdl.handle.net/10347/32669
UL http://hdl.handle.net/10347/32669
LA eng
NO Díaz, J. M., Prieto, A., López-Lorenzo, G., López-Novo, C., Iglesias, A., Díaz, P., ... & Fernández, G. (2019). Monitoring of the shedding and serological dynamics of Bovine gammaherpesvirus type 4 in a dairy cattle herd. Veterinary microbiology, 239, 108495
NO This work was supported by the Program for consolidating and structuring competitive research groups (GRC2015/003, Xunta de Galicia, Spain), by the Research Project ‘RUMIGAL: Rede de estudo multidisciplinar dos ruminantes en Galicia’ (R2014/005, Xunta de Galicia, Spain) and by a predoctoral grant to J.M. Díaz (Plan I2C, Xunta de Galicia, Spain).
DS Minerva
RD 24 abr 2026