RT Journal Article
T1 On the structure and stability of novel cationic DPPC liposomes doped with gemini surfactants
A1 Domínguez Arca, Vicente
A1 Sabín Fernández, Juan Daniel
A1 García Río, Luis
A1 Bastos, Margarida
A1 Taboada Antelo, Pablo
A1 Barbosa Fernández, Silvia
A1 Prieto Estévez, Gerardo
K1 Gemini surfactants
K1 Cationic liposomes
K1 Trans-gauche isomerization
K1 Lambda transitions
K1 Lipid-surfactant interactions
AB A novel formulation of cationic liposomes was studied by mixing dipalmitoylphosphatidylcholine (DPPC) with tetradecyltrimethylammonium bromide gemini surfactants with different alkane spacer groups lengths attached to their ammonium head-groups. The physicochemical characterization of the cationic liposomes was obtained by combining experimental results from differential scanning microcalorimetry (DSC) with molecular dynamic simulations, in order to understand their structural configuration. An adapted Ising model was used to interpret the results in terms of cooperativity of the phase transitions.The gemini surfactants partition into the lipid bilayer of DPPC liposomes, and the induced changes in colloidal stability and phase transition were analyzed in detail. The DPPC liposomes became positively charged upon gemini surfactant partition, showing increased colloidal stability. Our results show significant differences in structural configuration between gemini surfactants with short and long spacer lengths. While gemini with shorter spacers allocate within the lipid bilayer with both headgroups in the same layer, geminis with longer spacers unexpectedly intercalate in the lipid membrane in a particular zig-zag configuration, with each headgroup located at a different side of the bilayer, altering the coupling degree parameters of the membrane’s phase transition.The extraordinary increase of colloidal stability of DPPC liposomes with gemini surfactants at very low molar ratio and the possibility to tune the physicochemical properties of the membrane by control de spacer length of the geminis opens new possibilities for cationic liposomal formulations with potential applications in vaccines, drug/gene delivery or biosensing
PB Elsevier
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29452
UL http://hdl.handle.net/10347/29452
LA eng
NO Journal of Molecular Liquids 366 (2022) 120230
NO This work was supported by the Spanish Research Agency (AEI) under Project PID2019-109517RB-I00. ERDF funds are also acknowledged. Facilities provided by the Galician Supercomputing Centre (CESGA) are also acknowledged
DS Minerva
RD 25 abr 2026