RT Journal Article
T1 Combining DOE with neurofuzzy logic for healthy mineral nutrition of pistachio rootstocks in vitro culture
A1 Nezami Alanagh, Esmaeil
A1 Garoosi, Ghasem-Ali
A1 Landín Pérez, Mariana
A1 Gallego, Pedro Pablo
K1 DOE
K1 Micropropagation
K1 Health shoots
K1 Neurofuzzy logic
K1 Pistachio rootstock
K1 Physiological disorders
AB The aim of this study was to determine the effects of Murashige and Skoog (MS) salts on optimal growth of two pistachio rootstocks, P. vera cv. “Ghazvini” and “UCB1” using design of experiments (DOE) and artificial intelligence (AI) tools. MS medium with 14 macro—and micro-elements was used as base point and its concentration varied from 0 to 5 × MS concentrations. Design of experiments (DOE) software was used to generate a five-dimensional design space by categorizing MS salts into five independent factors (NH4NO3, KNO3, mesos, micros and iron), reducing the experimental design space from 3,125 to just 29 treatments. Typical plant growth parameters such as shoot quality (SQ), proliferation rate (PR), shoot length (SL), and some physiological disorders including shoot-tip necrosis (STN) and callus formation at the base of explants (BC) were evaluated for each treatment. The results were successfully modeled using neurofuzzy logic software. The model delivered new insights, by different sets of “IF–THEN” rules, pinpointing the key role of some ion interactions (SO42- × Cl−, K+ × SO42- × EDTA−, and Fe2+ × Cu2+ × NO3-) for SQ, PR, and SL, whilst physiological disorders (STN and BC) were governed mainly by independent ions as Fe2+ and EDTA−, respectively. In our opinion, the methodology and results obtained in this study is extremely useful to understand the effect of mineral nutrients on pistachio in vitro culture, through discovering new complex interactions among macro—and micro-elements which can be implemented to design new media of plant tissue culture and improve healthy plant micropropagation for any plant species
PB Fontiers Media
YR 2018
FD 2018
LK http://hdl.handle.net/10347/22504
UL http://hdl.handle.net/10347/22504
LA eng
NO Nezami-Alanagh E, Garoosi G-A, Landín M and Gallego PP (2018) Combining DOE With Neurofuzzy Logic for Healthy Mineral Nutrition of Pistachio Rootstocks in vitro Culture. Front. Plant Sci. 9:1474. doi: 10.3389/fpls.2018.01474
NO An especial thanks also goes to Xunta de Galicia of Spain, for financial support (Competitive Reference Groups, ED431C2017/09-FEDER and Programa REDES ED431D-2017/18, respectively)
DS Minerva
RD 25 abr 2026