RT Journal Article
T1 A Useful SNP Panel to Distinguish Two Cockle Species, Cerastoderma edule and C. glaucum, Co-Occurring in Some European Beds, and Their Putative Hybrids
A1 Maroso, Francesco
A1 Pérez de Gracia Pérez, Celia
A1 Iglesias Estepa, David
A1 Cao Hermida, María Asunción
A1 Díaz Costas, Seila María
A1 Villalba García, Antonio
A1 Vera Rodríguez, Manuel
A1 Martínez Portela, Paulino
K1 2b–RAD
K1 Cockles
K1 Diagnostic SNPs
K1 Hybrids
K1 New Generation Sequencing (NGS)
K1 SNaPshot
K1 Wildlife forensic
AB Cockles are highly appreciated mollusks and provide important services in coastal areas. The two European species, edible (Cerastoderma edule) and lagoon (Cerastoderma glaucum) cockles, are not easily distinguishable, especially when young. Interestingly, the species show different resistance to Marteilia cochillia, the parasite responsible for marteiliosis outbreaks, which is devastating cockle production in some areas. C. edule is severely affected by the parasite, while C. glaucum seems to be resistant, although underlying reasons are still unknown. Hybrids between both species might be interesting to introgress allelic variants responsible for tolerance, either naturally or through artificial selection, from lagoon into edible cockle. Here, we used 2b restriction site-associated DNA sequencing (2b–RAD) to identify single nucleotide polymorphisms (SNP) diagnostic for cockle discrimination (fixed for alternative allelic variants). Among the nine diagnostic SNPs selected, seven were validated using a SNaPshot assay in samples covering most of the distribution range of both species. The validated SNPs were used to check cockles that were suggested to be hybrids by a claimed diagnostic tool based on the internal transcribed spacers of the ribosomal RNA. Although these were shown to be false positives, we cannot rule out the fact that hybrids can occur and be viable. The SNP tool here developed will be valuable for their identification and management.
PB MDPI
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21878
UL http://hdl.handle.net/10347/21878
LA eng
NO Maroso, F.; Pérez de Gracia, C.; Iglesias, D.; Cao, A.; Díaz, S.; Villalba, A.; Vera, M.; Martínez, P. A Useful SNP Panel to Distinguish Two Cockle Species, Cerastoderma edule and C. glaucum, Co-Occurring in Some European Beds, and Their Putative Hybrids. Genes 2019, 10, 760
NO This research funded by INTERREG EUROPEAN PROGRAMME, grant number EAPA_458/2016 (COCKLES project)
DS Minerva
RD 28 abr 2026