RT Journal Article
T1 Single‑cell ICP‑MS for studying the association of inorganic  nanoparticles with cell lines derived from aquaculture species
A1 Suárez Oubiña, Cristian
A1 Herbello Hermelo, Paloma
A1 Mallo Seijas, Natalia
A1 Vázquez Ruiz de Ocenda, María
A1 Cabaleiro, Santiago
A1 Pinheiro, Ivone
A1  Rodríguez Lorenzo, Laura
A1  Espiña, Begoña
A1 Bermejo Barrera, Pilar
A1 Moreda Piñeiro, Antonio
K1 ScICP-MS
K1 Ammonia-based reaction cell
K1 Titanium dioxide nanoparticles
K1 Silver nanoparticles
K1 Nanoparticle uptake in cells
K1 Aquaculture species
AB The current research deals with the use of single-cell inductively coupled plasma-mass spectrometry (scICP-MS) for the assessment of titanium dioxide nanoparticle (TiO2 NP) and silver nanoparticle (Ag NP) associations in cell lines derived from aquaculture species (sea bass, sea bream, and clams). The optimization studies have considered the avoidance of high dissolved background, multi-cell peak coincidence, and possible spectral interferences. Optimum operating conditions were found when using a dwell time of 50 μs for silver and 100 μs for titanium. The assessment of associated TiO2 NPs by scICP-MS required the use of ammonia as a reaction gas (flow rate at 0.75 mL min−1) for interference-free titanium determinations (measurements at an m/z ratio of 131 from the 48Ti(NH)(NH3)4 adduct). The influence of other parameters such as the number of washing cycles and the cell concentration on accurate determinations by scICP-MS was also fully investigated. Cell exposure trials were performed using PVP-Ag NPs (15 and 100 nm, nominal diameter) and citrate-TiO2 NPs (5, 25, and 45 nm, nominal diameter) at nominal concentrations of 10 and 50 μg mL−1 for citrate-TiO2 NPs and 5.0 and 50 μg mL−1 for PVP-Ag NPs. Results have shown that citrate-TiO2 NPs interact with the outer cell membranes, being quite low in the number of citrate-TiO2 NPs that enters the cells (the high degree of aggregation is the main factor which leads to the aggregates being in the extracellular medium). In contrast, PVP-Ag NPs have been found to enter the cells
PB Springer
SN 1618-2642
YR 2023
FD 2023
LK http://hdl.handle.net/10347/30849
UL http://hdl.handle.net/10347/30849
LA eng
NO Suárez-Oubiña, C., Herbello-Hermelo, P., Mallo, N. et al. Single-cell ICP-MS for studying the association of inorganic nanoparticles with cell lines derived from aquaculture species. Anal Bioanal Chem 415, 3399–3413 (2023). https://doi.org/10.1007/s00216-023-04723-6
NO Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. European Union – Interreg POCTEP (ACUINANO, reference 07-12-ACUINANO_1_E); Ministerio de Economía y Competitividad (FOODNANORISK, reference PID2021-125276NB-I00); and Xunta de Galicia (Grupo de Referencia Competitiva, reference ED431C 2022/029)
DS Minerva
RD 1 may 2026