RT Journal Article
T1 Inhibition of Steptococcus mutans biofilm formation by extracts of Tenacibaculum sp. 20J, a bacterium with wide-spectrum quorum quenching activity
A1 Muras Mora, Andrea
A1 Mayer Mayer, Celia
A1 Romero Bernárdez, Manuel
A1 Camino Martínez, Tamara
A1 Ferrer, Maria D.
A1 Mira, Alex
A1 Otero Casal, Ana María
K1 Quorum sensing
K1 Quorum quenching
K1 AI-2
K1 Biofilms
K1 Dental plaque
K1 Streptococci
AB Background: Previous studies have suggested the quorum sensing signal AI-2 as a potential target to prevent the biofilm formation by Streptococcus mutans, a pathogen involved in tooth decay.Objective: To obtain inhibition of biofilm formation by S. mutans by extracts obtained from the marine bacterium Tenacibaculum sp. 20J interfering with the AI-2 quorum sensing system.Design: The AI-2 inhibitory activity was tested with the biosensors Vibrio harveyi BB170 and JMH597. S. mutans ATCC25175 biofilm formation was monitored using impedance real-time measurements with the xCELLigence system®, confocal laser microscopy, and the crystal violet quantification method.Results: The addition of the cell extract from Tenacibaculum sp. 20J reduced biofilm formation in S. mutans ATCC25175 by 40–50% compared to the control without significantly affecting growth. A decrease of almost 40% was also observed in S. oralis DSM20627 and S. dentisani 7747 biofilms.Conclusions: The ability of Tenacibaculum sp. 20J to interfere with AI-2 and inhibit biofilm formation in S. mutans was demonstrated. The results indicate that the inhibition of quorum sensing processes may constitute a suitable strategy for inhibiting dental plaque formation, although additional experiments using mixed biofilm models would be required
PB Taylor & Francis
YR 2018
FD 2018-01-30
LK http://hdl.handle.net/10347/17648
UL http://hdl.handle.net/10347/17648
LA eng
NO Muras, A., Mayer, C., Romero, M., Camino, T., Ferrer, M., Mira, A., & Otero, A. (2018). Inhibition of Steptococcus mutans biofilm formation by extracts of Tenacibaculum sp. 20J, a bacterium with wide-spectrum quorum quenching activity. Journal Of Oral Microbiology, 10(1), 1429788. doi: 10.1080/20002297.2018.1429788
NO This work was supported by the non-profit organization Fundación Ramón Areces [CIVP16A1814] and by the grant ‘Axudas do Programa de Consolidación e Estructuración de Unidades de Investigación Competitivas (GPC)’ from the Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia (ED431B2017/53). A.M. was supported by a predoctoral fellowship from the Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia (ED481A2015/311)
DS Minerva
RD 30 abr 2026