RT Journal Article
T1 In Vivo Theranostics at the Peri-Infarct Region in Cerebral Ischemia
A1 Agulla Freire, Jesús
A1 Brea López, David
A1 Campos Pérez, Francisco
A1 Sobrino Moreiras, Tomás
A1 Argibay González, Bárbara
A1 Al-Soufi, Wajih
A1 Blanco González, Miguel
A1 Castillo Sánchez, José Antonio
A1 Ramos Cabrer, Pedro
K1 Cerebral ischemia
K1 Peri-infarct region
K1 MRI
K1 Theranostics
K1 Drug delivery
AB The use of theranostics in neurosciences has been rare to date because of the limitations imposed on the free delivery of substances to the brain by the blood-brain barrier. Here we report the development of a theranostic system for the treatment of stroke, a leading cause of death and disability in developed countries. We first performed a series of proteomic, immunoblotting and immunohistological studies to characterize the expression of molecular biomarkers for the so-called peri-infarct tissue, a key region of the brain for stroke treatment. We confirmed that the HSP72 protein is a suitable biomarker for the peri-infarct region, as it is selectively expressed by at-risk tissue for up to 7 days following cerebral ischemia. We also describe the development of anti-HSP72 vectorized stealth immunoliposomes containing imaging probes to make them traceable by conventional imaging techniques (fluorescence and MRI) that were used to encapsulate a therapeutic agent (citicoline) for the treatment of cerebral ischemia. We tested the molecular recognition capabilities of these nano-platforms in vitro together with their diagnostic and therapeutic properties in vivo, in an animal model of cerebral ischemia. Using MRI, we found that 80% of vectorized liposomes were located on the periphery of the ischemic lesion, and animals treated with citicoline encapsulated on these liposomes presented lesion volumes up to 30% smaller than animals treated with free (non-encapsulated) drugs. Our results show the potential of nanotechnology for the development of effective tools for the treatment of neurological diseases
PB Ivyspring International Publisher
SN 1838-7640
YR 2014
FD 2014
LK http://hdl.handle.net/10347/17075
UL http://hdl.handle.net/10347/17075
LA eng
NO Agulla J, Brea D, Campos F, Sobrino T, Argibay B, Al-Soufi W, Blanco M, Castillo J, Ramos-Cabrer P. In Vivo Theranostics at the Peri-Infarct Region in Cerebral Ischemia. Theranostics 2014; 4(1):90-105. doi:10.7150/thno.7088. Available from http://www.thno.org/v04p0090.htm
NO Financial support from the Instituto de Salud Carlos III of the Spanish Ministry of Health (projects PI11/02161 and CP09/00074), the Spanish Ministry of Economy and Competence (projects SAF2008-02190, SAF2011-30517 and RETICS-INVICTUS R012/0014), and the European Union (FEDER program) is gratefully acknowledged. Research contracts from the Miguel Servet program (PRC and TS) and the Sara Borrell program (JA and DB) of the Instituto de Salud Carlos III are acknowledged
DS Minerva
RD 23 abr 2026