RT Journal Article
T1 Microsatellite marker development in the protozoan parasite Perkinsus olseni
A1 Gómez Pardo, María Belén
A1 Cao Hermida, María Asunción
A1 Vilas Peteiro, Román
A1 Abollo, Elvira
A1 Villalba García, Antonio
A1 Martínez Portela, Paulino
K1 Microsatellites
K1 Perkinsus olseni
K1 Genetic diversity
K1 Clams
K1 Diploidy
K1 Protozoan
K1 Parasite
AB The analysis of an enriched partial genomic library and of public expressed sequence tag (EST) resources allowed the characterization of the first microsatellite loci in the protozoan parasite Perkinsus olseni. Clonal cultures from laboratory isolates derived from infected clams Ruditapes decussatus (from Spain), R. philippinarum (from Spain and Japan), and Austrovenus stutchburyi (from New Zealand) were used for the characterization of 12 microsatellites. Low variation was detected at most loci, with the number of alleles at polymorphic loci ranging from 2 to 7 (average 3.20 ± 0.51) and gene diversity from 0.11 to 0.79 (average 0.40 ± 0.07). Preliminary results show that (1) isolates of P. olseni are diploid cells, and (2) multiple infections can occur within a single host. Eight of the loci analyzed successfully cross-amplified in the congeneric species P. mediterraneus. These microsatellite markers will be useful to analyze in detail the population genetic structure of P. olseni, crucial for the efficient management of this parasitic disease
PB Inter-Research
SN 0177-5103
YR 2011
FD 2011
LK http://hdl.handle.net/10347/26298
UL http://hdl.handle.net/10347/26298
LA eng
NO Pardo BG, Cao A, Vilas R, Abollo E, Villalba A, Martínez P (2011) Microsatellite marker development in the protozoan parasite Perkinsus olseni. Dis Aquat Org 94:161-165. https://doi.org/10.3354/dao02325
NO This study was supported by the Ministerio de Ciencia e Innovación of the Spanish government, under the project AGL2006-11809. B.G.P. and R.V. were supported by an Isidro Parga Pondal research fellowship from Xunta de Galicia (Spain); E.A. was supported by a Ramón y Cajal contract of the Ministerio de Ciencia e Innovación of the Spanish Government
DS Minerva
RD 23 abr 2026