RT Journal Article
T1 Modification of betanodavirus virulence by substitutions in the 3’ terminal region of RNA2
A1 Souto Pereira, Sandra
A1 Olveira Hermida, José Gabriel
A1 Pereira Dopazo, Carlos
A1 Borrego García, Juan José
A1 Bandín Matos, Isabel
K1 Interaction
K1 Reassortant betanodavirus
K1 RNA2
K1 Virulence attenuation
K1 RNA1
AB Betanodaviruses have bi-segmented positive-sense RNA genomes, consisting of RNAs 1 and 2. For some members of the related genus alphanodavirus, the 3' terminal 50 nucleotides (nt) of RNA2, including a predicted stem-loop structure (3'SL), are essential for replication. We investigate the possible existence and role of a similar structure in a reassortant betanodavirus strain (RGNNV/SJNNV). In this study, we developed three recombinant strains containing nucleotide changes at positions 1408 and 1412. Predictive models showed stem-loop structures involving nt 1398–1421 of the natural reassortant whereas this structure is modified in the recombinant viruses harbouring point mutations r1408 and r1408– 1412, but not in r1412. Results obtained from infectivity assays showed differences between the reference strains and the mutants in both RNA1 and RNA2 synthesis. Moreover, an imbalance between the synthesis of both segments was demonstrated, mainly with the double mutant. All these results suggest an interaction between RNA1 and the 3' non-coding regions (3¢NCR) of RNA2. In addition, the significant attenuation of the virulence for Senegalese sole and the delayed replication of r1408–1412 in brain tissues may point to an interaction of RNA2 with host cellular proteins
PB Microbiology Society
SN 0022-1317
YR 2018
FD 2018
LK http://hdl.handle.net/10347/17440
UL http://hdl.handle.net/10347/17440
LA eng
NO Souto, S., Olveira, J., Dopazo, C., Borrego, J., & Bandín, I. (2018). Modification of betanodavirus virulence by substitutions in the 3' terminal region of RNA2. Journal Of General Virology, 99(9), 1210-1220. doi: 10.1099/jgv.0.001112
NO This work was supported by grant AGL2014-54532-C2-2-R from the Ministerio de Innovación y Competitividad (Spain), co-funded by FEDER
DS Minerva
RD 24 abr 2026