RT Book,_Section T1 Philasterides dicentrarchi A1 Lamas Fernández, Jesús A1 Leiro Vidal, José Manuel A2 Sitjà-Bobadilla, Ariadna A2 Bron, James E. A2 Wiegertjes, Geert A2 Piazzon, M. Carla K1 Philasterides dicentrarchi K1 Isolation K1 Culture K1 Transmission K1 Freezing methodology AB In this book chapter, we describe the equipment and methodologies used to isolate and culture the fish parasite Philasterides dicentrarchi, as well as the freezing techniques employed to maintain the parasite for extended periods. We also outline the procedures for transmission, infection, and vaccine preparation. PB 5M Books SN 9781789181333 YR 2021 FD 2021 LK https://hdl.handle.net/10347/44921 UL https://hdl.handle.net/10347/44921 LA eng NO Lamas, J., Leiro, J.M. (2021). Philasterides dicentrarchi. In: A. Sitjà-Bobadilla et al. (eds.). Fish parasites. A Handbook of Protocols for their Isolation, Culture and Transmission. 5M Books. 9781789181333 (pp. 33-48) NO Some of the research reported in this chapter was financially sup- ported by the PARAFISHCONTROL project, which received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 634429, by grant AGL2017–83577-R awarded by the Ministerio de Economía y Competitividad (Spain) and Fondo Europeo de Desarrollo Regional – FEDER – (European Union), and by grant ED431C2017/31 from the Xunta de Galicia (Spain). DS Minerva RD 28 abr 2026