RT Journal Article
T1 High-affinity A/T-rich DNA binding with a dimeric bisbenzamidine
A1 Bouzada Reboredo, David
A1 Paul, Ananya
A1 Sánchez López, Mateo Isidro
A1 Domínguez-Asenjo, Bárbara
A1 Lence Quintana, Emilio José
A1 Melle-Franco, Manuel
A1 Ardá, Ana
A1 Barreiro Piñeiro, Natalia
A1 Penas, Cristina
A1 Gómez González, Jacobo
A1 Jiménez-Barbero, Jesús
A1 Moreno, Javier
A1 González Bello, Concepción
A1 Wilson, W. David
A1 Mascareñas Cid, José Luis
A1 Vázquez Sentís, Marco Eugenio
K1 Dimeric bisbenzamidine
K1 ADN
K1 A/T
AB A bisbenzamidine DNA binding agent can be easily dimerized by alkylation of its terminal amidine groups to afford an extended minor groove binder with over 20-fold enhanced DNA affinity toward extended A/T-rich sites. Nuclear magnetic resonance experiments in combination with molecular dynamics simulation studies provide structural insight into the insertion of this compound in the DNA minor groove, and antimicrobial assays demonstrate that the increased affinity translates into higher antileishmanial activity.
PB Oxford Academic
SN 2976-856X
YR 2024
FD 2024
LK https://hdl.handle.net/10347/40115
UL https://hdl.handle.net/10347/40115
LA eng
NO Bouzada, D., Paul, A., Sánchez, M. I., Domínguez-Asenjo, B., Lence, E., Melle-Franco, M., Ardá, A., Barreiro-Piñeiro, N., Penas, C., Gómez-González, J., Jiménez-Barbero, J., Moreno, J., González-Bello, C., Wilson, W. D., Mascareñas, J. L., & Vázquez, M. E. (2024). High-affinity A/T-rich DNA binding with a dimeric bisbenzamidine.  NAR Molecular Medicine, 1(4), Article ugae022 .  https://doi.org/10.1093/narmme/ugae022
NO Agencia Estatal de Investigación [RTI2018-099877-B-I00, PID2022-136963OB-I00, PID2021-127702NB-I00]; Xunta de Galicia [ED431C 2021/29, ED431G 2023/03]; European Regional Development Fund; CICECO [IF/00894/2015, UIDB/50011/2020, UIDP/50011/2020, LA/P/0006/2020]; EDCTP [RIA2020S-3301]; ISCIII [PI21CIII/00005]; CIBERINFEC [CB21/13/00018].
DS Minerva
RD 22 abr 2026