RT Journal Article
T1 Presence of an isoform of H+-pyrophosphatase located in the alveolar sacs of a scuticociliate parasite of turbot: physiological consequences
A1 Mallo Seijas, Natalia
A1 Lamas Fernández, Jesús
A1 Felipe González, Ana Paula de
A1 Castro de Antonio, María Eugenia de
A1 Sueiro Benavides, Rosa Ana
A1 Leiro Vidal, José Manuel
K1 H+-PPase
K1 Philasterides dicentrarchi
K1 Alveolar sacs
K1 Osmoregulation
AB H+-pyrophosphatases (H+-PPases) are integral membrane proteins that couple pyrophosphate energy to an electrochemical gradient across biological membranes and promote the acidification of cellular compartments. Eukaryotic organisms, essentially plants and protozoan parasites, contain various types of H+-PPases associated with vacuoles, plasma membrane and acidic Ca+2 storage organelles called acidocalcisomes. We used Lysotracker Red DND-99 staining to identify two acidic cellular compartments in trophozoites of the marine scuticociliate parasite Philasterides dicentrarchi: the phagocytic vacuoles and the alveolar sacs. The membranes of these compartments also contain H+-PPase, which may promote acidification of these cell structures. We also demonstrated for the first time that the P. dicentrarchi H+-PPase has two isoforms: H+-PPase 1 and 2. Isoform 2, which is probably generated by splicing, is located in the membranes of the alveolar sacs and has an amino acid motif recognized by the H+-PPase-specific antibody PABHK. The amino acid sequences of different isolates of this ciliate are highly conserved. Gene and protein expression in this isoform are significantly regulated by variations in salinity, indicating a possible physiological role of this enzyme and the alveolar sacs in osmoregulation and salt tolerance in P. dicentrarchi
PB Cambridge University Press
SN 0031-1820
YR 2016
FD 2016-04
LK http://hdl.handle.net/10347/18171
UL http://hdl.handle.net/10347/18171
LA eng
NO Mallo N, Lamas J, Defelipe AP, Decastro ME, Sueiro RA, Leiro JM. (2016).Presence of an isoform of H+-pyrophosphatase located in the alveolar sacs of a scuticociliate parasite of turbot: physiological consequences. Parasitology143: 576-87. doi: 10.1017/S0031182015001997
NO This article has been published in a revised form in Parasitology[http://doi.org/10.1017/ S0031182015001997]. This version is free to viewand download for private research and study only. Not for re-distribution,re-sale or use in derivative works. © 2017 Cambridge University Press
NO This work was financially supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 634429 (PARAFISHCONTROL),by the Ministerio de Economía y Competitividad  (Spain) under grant agreement AGL2014-57125-R and by grant GPC2014/069 from the Xunta de Galicia (Spain)
DS Minerva
RD 22 abr 2026