RT Journal Article
T1 Production of microbial transglutaminase on media made from sugar cane molasses and glycerol
A1 Portilla Rivera, Óscar M.
A1 Téllez Luis, Simón J.
A1 Ramírez de León, José Alberto
A1 Vázquez Vázquez, Manuel
K1 Transglutaminase
K1 Glycerol
K1 Glucose
K1 Sugar cane molasses
AB Transglutaminase is an enzyme that catalyses an acyl transfer reaction between γ-carboxamide groups of glutaminyl residues and lysine residues in proteins. Due to this property, this enzyme is used for enhancing textural properties of protein-rich food. The transglutaminase used as food additive is obtained by microorganisms, mainly by Streptoverticillium ladakanum. On the other hand, sugar cane molasses is a viscous liquid rich in noncrystallized carbohydrates (saccharose, glucose and fructose). In this work, the feasibility of using sugar cane molasses as a carbon source for the production of microbial transglutaminase by Streptoverticillium ladakanum NRRL 3191 has been studied. Carbon sources including sugar cane molasses (60 g of total sugars per L), glycerol (60 g/L) and their mixture in a ratio of 1:1 (30 g/L of each) were evaluated. Time course of microbial growth, transglutaminase activity and carbon source consumption were determined every 24 h during 120 h of fermentations at three agitation speeds (200, 300 or 400 rpm). The results showed that with the increase in agitation speed, the biomass concentration increased up to 8.39 g/L in the medium containing sugar cane molasses alone or the mixture of molasses and glycerol. The highest transglutaminase activity was obtained at 400 rpm in the medium containing a mixture of molasses and glycerol, reaching 0.460 U/mL, while in the medium containing sugar cane molasses alone, the activity was 0.240 U/mL, and using glycerol alone it was 0.250 U/mL. These results show that sugar cane molasses is a suitable medium for transglutaminase production when it is combined with glycerol
PB University of Zagreb
SN 1330-9862
YR 2009
FD 2009
LK http://hdl.handle.net/10347/23048
UL http://hdl.handle.net/10347/23048
LA eng
NO Portilla Rivera, Oscar M. ; Téllez Luis, Simón J. ; Ramírez de León, José A. ; Vázquez, Manuel. (2009). Production of Microbial Transglutaminase. "Food Technology Biotechnology", vol. 47, n. 1, p. 19–26
NO A grant from FOMIX CONACYT – Gobierno de Tamaulipas (Ref. 2004/1055) to author Portilla-Rivera is gratefully acknowledged. The authors are grateful to Ministerio de Educación y Ciencia (Spain) for the financial support of this work (Project: AGL2006-08250/ALI)
DS Minerva
RD 25 abr 2026