RT Journal Article
T1 A Novel Nanoproteomic Approach for the Identification of Molecular Targets Associated with Thyroid Tumors
A1 García Vence, María
A1 Chantada Vázquez, María del Pilar
A1 Cameselle Teijeiro, José Manuel
A1 Bravo López, Susana Belén
K1 Gold nanoparticles (AuNPs)
K1 Silver nanoparticles (AgNPs)
K1 Magnetic nanoparticles (FeNPs)
K1 Protein corona (PC)
K1 Thyroid cancer
K1 Molecular target
AB A thyroid nodule is the most common presentation of thyroid cancer; thus, it is extremely important to differentiate benign from malignant nodules. Within malignant lesions, classification of a thyroid tumor is the primary step in the assessment of the prognosis and selection of treatment. Currently, fine-needle aspiration biopsy (FNAB) is the preoperative test most commonly used for the initial thyroid nodule diagnosis. However, due to some limitations of FNAB, different high-throughput “omics” approaches have emerged that could further support diagnosis based on histopathological patterns. In the present work, formalin-fixed paraffin-embedded (FFPE) tissue specimens from normal (non-neoplastic) thyroid (normal controls (NCs)), benign tumors (follicular thyroid adenomas (FTAs)), and some common types of well-differentiated thyroid carcinoma (follicular thyroid carcinomas (FTCs), conventional or classical papillary thyroid carcinomas (CV-PTCs), and the follicular variant of papillary thyroid carcinomas (FV-PTCs)) were analyzed. For the first time, FFPE thyroid samples were deparaffinized using an easy, fast, and non-toxic method. Protein extracts from thyroid tissue samples were analyzed using a nanoparticle-assisted proteomics approach combined with shotgun LC-MS/MS. The differentially regulated proteins found to be specific for the FTA, FTC, CV-PTC, and FV-PTC subtypes were analyzed with the bioinformatic tools STRING and PANTHER showing a profile of proteins implicated in the thyroid cancer metabolic reprogramming, cancer progression, and metastasis. These proteins represent a new source of potential molecular targets related to thyroid tumors
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/24021
UL http://hdl.handle.net/10347/24021
LA eng
NO García-Vence, M.; Chantada-Vázquez, M.P.; Cameselle-Teijeiro, J.M.; Bravo, S.B.; Núñez, C. A Novel Nanoproteomic Approach for the Identification of Molecular Targets Associated with Thyroid Tumors. Nanomaterials 2020, 10, 2370
NO This work was supported by grants from the Instituto de Salud Carlos III (ISCIII), State Research Agency (AEI), and Ministry of Science and Innovation (Spain), with the participation of European FEDER funds, to C.N. (CP16/00139) and J.M.C.-T. (PI19/01316)
DS Minerva
RD 22 abr 2026