RT Journal Article
T1 miRNome Characterization of Milk-Derived Extracellular Vesicles in Recombinant Somatotropin-Treated Dairy Cows
A1 Benedetto, Alessandro
A1 Giaccio, Nunzia
A1 Arigoni, Maddalena
A1 Calogero, Raffaele Adolfo
A1 Regal López, Patricia
A1 Lamas Freire, Alexandre
A1 Martucci, Francesca
A1 Audino, Valentina
A1 Dervilly, Gaud
A1 Pezzolato, Marzia
A1 Bozzetta, Elena
K1 Milk
K1 miRNA
K1 Recombinant somatotropin
K1 Extracellular vesicles
K1 Untargeted transcriptomics
K1 Biomarker
AB The recombinant bovine somatotropin (rbST) is a synthetic hormone developed to mimic the effects of the endogenous growth hormone, also known as bovine somatotropin (bST). Although rbST use in dairy cows is authorized in several countries, it is currently banned in Europe. Different methods for screening and confirmatory detection of rbST were developed, mainly based on LC-MS/MS and immune-enzymatic assays. However, some commercial forms of rbST have above the same amino acid sequence of bST, making it difficult to produce a reliable differentiation of recombinant from endogenous forms. Complementary strategies for indirect detection of rbST can therefore be considered as alternative biomarker-based tools. Untargeted transcriptomics was applied to characterize the microRNAs (miRNA) isolated from milk extracellular vesicles (EVs) in rbST-treated animals, aiming the identification of non-coding biomarkers related to its administration. Sequencing analysis of 63 archive samples collected during previous animal trial allowed for the identification of 35 differentially expressed (DE) miRNAs. A validation study performed by qPCR on a further 70 milk samples from a field survey confirmed the significant upregulation of bta-miR-10167-3p in milk EV from rbST-treated cows. The results obtained suggest the potential use of bta-miR-10167-3p as a non-invasive biomarker to be considered in novel screening strategies, needed to tackle rbST misuse in dairy cows.
PB MDPI
SN 1422-0067
YR 2025
FD 2025-03-08
LK https://hdl.handle.net/10347/43447
UL https://hdl.handle.net/10347/43447
LA eng
NO Benedetto, A.; Giaccio, N.; Arigoni, M.; Calogero, R.A.; Regal, P.; Lamas, A.; Martucci, F.; Audino, V.; Dervilly, G.; Pezzolato, M.; et al. miRNome Characterization of Milk-Derived Extracellular Vesicles in Recombinant Somatotropin-Treated Dairy Cows. Int. J. Mol. Sci. 2025, 26, 2437. https://doi.org/10.3390/ ijms26062437
NO IZS PLV 03/21 RC
DS Minerva
RD 22 abr 2026