RT Journal Article
T1 Sublingual boosting with a novel mucoadhesive thermogelling hydrogel following parenteral CAF01 priming as a strategy against Chlamydia trachomatis
A1 García del Rio, Lorena
A1 Díaz Rodríguez, Patricia
A1 Pedersen, Gabriel Kristian
A1 Christensen, Dennis
A1 Landín Pérez, Mariana
K1 Cationic adjuvant liposomes
K1 Mucoadhesive and thermosensitive platforms
K1 Mucosal vaccination
K1 Polymeric networks
K1 Prime-boost strategy
K1 Systemic and sublingual immunization
AB Chlamydia trachomatis is the most prevalent sexually transmitted disease of bacterial origin. The high number of asymptomatic cases makes it difficult to stop the transmission, requiring vaccine development. Herein, a strategy is proposed to obtain local genital tract immunity against C. trachomatis through parenteral prime and sublingual boost. Subcutaneous administration of chlamydia CTH522 subunit vaccine loaded in the adjuvant CAF01 is combined with sublingual administration of CTH522 loaded in a novel thermosensitive and mucoadhesive hydrogel. Briefly, a ternary optimized hydrogel (OGEL) with desirable biological and physicochemical properties is obtained using artificial intelligence techniques. This formulation exhibits a high gel strength and a strong mucoadhesive, adhesive and cohesive nature. The thermosensitive properties of the hydrogel facilitate application under the tongue. Meanwhile the fast gelation at body temperature together with rapid antigen release should avoid CTH522 leakage by swallowing and increase the contact with sublingual tissue, thus promoting absorption. In vivo studies demonstrate that parenteral-sublingual prime-boost immunization, using CAF01 and OGEL as CTH522 vaccine carriers, shows a tendency to increase cellular (Th1/Th17) immune responses when compared to mucosal or parenteral vaccination alone. Furthermore, parenteral prime with CAF01/CTH522 followed by sublingual boosting with OGEL/CTH522 elicits a local IgA response in the genital tract
PB Wiley
SN 2192-2640
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29227
UL http://hdl.handle.net/10347/29227
LA eng
NO Garcia‐del Rio, L., Diaz‐Rodriguez, P., Pedersen, G. K., Christensen, D., & Landin, M. (2022). Sublingual boosting with a novel mucoadhesive thermogelling hydrogel following parenteral CAF01 priming as a strategy against chlamydia trachomatis. Advanced Healthcare Materials, 11(11). doi:10.1002/adhm.202102508
NO European Commission-Interreg V-A POCTEP (0245_IBEROS_1_E) EU (FEDER). Xunta de Galicia (Competitive Reference Groups, ED43C2017/09-FEDER). European Comission-COST Action CA16231 “European Network of Vaccine Adjuvants” (ENOVA). European Commission-European Network of Vaccine Adjuvants STSM fellowship (ECOST-STSM-CA16231-251019-114264)
DS Minerva
RD 24 abr 2026