RT Journal Article
T1 Higher expression of Toll-like receptors 2 and 4 in blood cells of Keratoconus patiens
A1 Sobrino Moreiras, Tomás
A1 Regueiro, Uxía 
A1 Malfeito Jiménez, María de las Mercedes
A1 Vieites Prado, Alba
A1 Pérez Mato, María
A1 Campos Pérez, Francisco
A1 Lema Gesto, María Isabel
AB Inflammation may play a significant role in Keratoconus (KC), but the implication of immunity on this inflammatory response is unknown. Therefore, our aim was to determine the expression levels of Toll-like receptors 2 (TLR2) and 4 (TLR4) in monocytes and neutrophils from patients with KC and control subjects for demonstrating the role of innate immunity in KC. We also study the correlation between TLR2/TLR4 expression and serum levels of proinflammatory markers (IL-1β, IL-6, TNF-α, MMP-9 and NF-κB). Forty patients with bilateral KC (55% males; mean age; 33.1 ± 10.9 years) and 20 control subjects (55% males; mean age; 30.4 ± 7.6 years) were included in the study. Our results showed that mean expression of TLR2 and TLR4 in both neutrophils and monocytes was significantly higher in patients with KC compared to control subjects (all p < 0.0001). Furthermore, KC patients also showed higher serum levels of IL-1B, IL-6, TNF-α, MMP-9 (all p < 0.0001) and NF-κB (p = 0.036). In addition, we found a strong correlation between TLR2 expression in both monocytes and neutrophils (all p < 0.0001), and TLR4 in monocytes (all p < 0.05) with serum levels of IL-1B, IL-6, TNF-α and MMP-9. In conclusion, these findings suggest that TLRs may play an important role in the pathophysiology of KC.
PB Nature Publishing Group
YR 2017
FD 2017
LK http://hdl.handle.net/10347/22938
UL http://hdl.handle.net/10347/22938
LA eng
NO Sobrino, T., Regueiro, U., Malfeito, M. et al. Higher Expression of Toll-Like Receptors 2 and 4 in Blood Cells of Keratoconus Patiens. Sci Rep 7, 12975 (2017). https://doi.org/10.1038/s41598-017-13525-7
NO This project has been supported by grants from the Spanish Ministry of Economy and Competitiveness (Instituto de Salud Carlos III: PI14/00247); Xunta de Galicia (Consellería de Economía e Industria: 10 PX113918156PR and Consellería de Educación: GRC2014/027) and the European Union program FEDER. T. Sobrino (CP12/03121) and F. Campos (CP14/00154) are recipients of a research contract from Miguel Servet Program of Instituto de Salud Carlos III. Likewise A. Vieites-Prado is recipient of a FPI fellow (BES-2012-056027) from the Spanish Ministry of Economy and Competitiveness; and U. Regueiro is recipient of a predoctoral Fellow from the Xunta de Galicia (ED481A-2015/001)
DS Minerva
RD 24 abr 2026