RT Journal Article
T1 Fusogenic Cell-Derived nanocarriers for cytosolic delivery of cargo inside living cells
A1 Soprano, Enrica
A1 Migliavacca, Martina
A1 López Ferreiro, Miriam
A1 Pelaz García, Beatriz
A1 Polo Tobajas, Ester
A1 Pino González de la Higuera, Pablo Alfonso del
K1 Biomimetic
K1 Intracellular delivery
K1 Nanocarrier
K1 Fusogenic
K1 Cell membrane coating
AB A surface-engineered cell-derived nanocarrier was developed for efficient cytosolic delivery of encapsulated biologically active molecules inside living cells. Thus, a combination of aromatic-labeled and cationic lipids, instrumental in providing fusogenic properties, was intercalated into the biomimetic shell of self-assembled nanocarriers formed from cell membrane extracts. The nanocarriers were loaded, as a proof of concept, with either bisbenzimide molecules, a fluorescently labeled dextran polymer, the bicyclic heptapeptide phalloidin, fluorescently labeled polystyrene nanoparticles or a ribonucleoprotein complex (Cas9/sgRNA). The demonstrated nanocarrieŕs fusogenic behavior relies on the fusogen-like properties imparted by the intercalated exogenous lipids, which allows for circumventing lysosomal storage, thereby leading to efficient delivery into the cytosolic milieu where cargo regains function
PB Elsevier
SN 0021-9797
YR 2023
FD 2023-06-07
LK http://hdl.handle.net/10347/31054
UL http://hdl.handle.net/10347/31054
LA eng
NO Journal of Colloid and Interface Science 648 (2023) 488–496. https://doi.org/10.1016/j.jcis.2023.06.015
NO The authors thank the financial support of the European Research Council (starting grant #950421), the European Union (INTERREG V-A Spain–Portugal #0624_2IQBIONEURO_6_E, NextGenerationEU/PRTR and ERDF), the MCIN/AEI (PID2020-119206RB-I00, PID2020-119479RA-I00, PID2019-111218RB-I00, RYC-2017-23457 and RYC-2019-028238-I), and the Xunta de Galicia (ED431F 2021/02, 2021-CP090, ED431C 2022/018, and Centro Singular De Investigación de Galicia Accreditation 2019–2022 #ED431G 2019/03). This project was also supported by the ISCIII, under the framework of EuroNanoMed III_2020 (AC20/00041, PLATMED). We would also like to thank our colleagues Dr. M. Collado and Dr. M.A. Moreno-Mateos for their valuable insights and suggestions. We thank Dr. M. Collado (IDIS, Spain) for a gift of 293-T-HEK-dEGFP cells
DS Minerva
RD 26 abr 2026