RT Journal Article
T1 Sequence identification of bioactive peptides from amaranth seed proteins (Amaranthus hypochondriacus spp.)
A1 Ayala Niño, Alexis
A1 Rodríguez Serrano, Gabriela Mariana
A1 González Olivares, Luis Guillermo
A1 Contreras López, Elizabeth
A1 Regal López, Patricia
A1 Cepeda Sáez, Alberto
K1 Amaranth protein
K1 Flavourzyme
K1 Alcalase
K1 Bioactive peptides
K1 Hydrolysates
AB Amaranthus hypochondriacus spp. is a commonly grown cereal in Latin America, known for its high protein content. The objective of this study was to separate and identify bioactive peptides found in amaranth seeds through enzymatically-assisted hydrolysis using alcalase and flavourzyme. Hydrolysis was carried out for each enzyme separately and compared to two-step continuous process where both enzymes were combined. The biological activity of the resulting three hydrolysates was analyzed, finding, in general, higher bioactive potential of the hydrolysate obtained in a continuous process (combined enzymes). Its fractions were separated by RP-HPLC, and their bioactivity was analyzed. In particular, two fractions showed the highest biological activity as ACE inhibitors with IC50 at 0.158 and 0.134, thrombin inhibitors with IC50 of 167 and 155, and antioxidants in ABTS assay with SC50 at 1.375 and 0.992 mg/L, respectively. Further sequence analysis of the bioactive peptides was carried out using MALDI-TOF, which identified amino acid chains that have not been reported as bioactive so far. Bibliographic survey allowed identification of similarities between peptides reported in amaranth and other proteins. In conclusion, amaranth proteins are a potential source of peptides with multifunctional activity.
PB MDPI
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21157
UL http://hdl.handle.net/10347/21157
LA eng
NO Ayala-Niño, A.; Rodríguez-Serrano, G.M.; González-Olivares, L.G.; Contreras-López, E.; Regal-López, P.; Cepeda-Saez, A. Sequence Identification of Bioactive Peptides from Amaranth Seed Proteins (Amaranthus hypochondriacus spp.). Molecules 2019, 24, 3033
DS Minerva
RD 27 abr 2026