RT Journal Article
T1 Cumulative acquisition of pathogenicity islands has shaped virulence potential and contributed to the emergence of LEE-negative Shiga toxinproducing Escherichia coli strains
A1 Montero, David Arturo
A1 Canto, Felipe del
A1 Velasco, Juliana
A1 Colello, Rocío
A1 Padola, Nora Lía
A1 Salazar, Juan Carlos
A1 San Martín, Carla
A1 Oñate, Ángel
A1 Blanco Álvarez, Jorge
A1 Rasko, David A.
A1 Contreras, Carmen
A1 Puente, José Luis
A1 Scheutz, Flemming
A1 Franz, Eelco
A1 Vidal, Roberto M.
K1 LEE-negative STEC
K1 Pathogenicity island
K1 Comparative genomics
K1 Integrative conjugative element
K1 Locus of adhesion and autoaggregation
AB Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens causing severe gastroenteritis, which may lead tohemolytic uremic syndrome. The Locus of Enterocyte Effacement (LEE), a Pathogenicity Island (PAI), is a majordeterminant of intestinal epithelium attachment of a group of STEC strains; however, the virulence repertoire of STECstrains lacking LEE, has not been fully characterized. The incidence of LEE-negative STEC strains has increased inseveral countries, highlighting the relevance of their study. In order to gain insights into the basis for the emergenceof LEE-negative STEC strains, we performed a large-scale genomic analysis of 367 strains isolated worldwide fromhumans, animals, food and the environment. We identified uncharacterized genomic islands, including two PAIs andone Integrative Conjugative Element. Additionally, the Locus of Adhesion and Autoaggregation (LAA) was the mostprevalent PAI among LEE-negative strains and we found that it contributes to colonization of the mice intestine. Ourcomprehensive and rigorous comparative genomic and phylogenetic analyses suggest that the accumulativeacquisition of PAIs has played an important, but currently unappreciated role, in the evolution of virulence in thesestrains. This study provides new knowledge on the pathogenicity of LEE-negative STEC strains and identifies molecularmarkers for their epidemiological surveillance.
PB Taylor & Francis
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21397
UL http://hdl.handle.net/10347/21397
LA eng
NO David Arturo Montero, Felipe Del Canto, Juliana Velasco, Rocío Colello, Nora Lia Padola, Juan Carlos Salazar, Carla San Martin, Angel Oñate, Jorge Blanco, David A. Rasko, Carmen Contreras, Jose Luis Puente, Flemming Scheutz, Eelco Franz & Roberto M. Vidal (2019) Cumulative acquisition of pathogenicity islands has shaped virulence potential and contributed to the emergence of LEE-negative Shiga toxin-producing Escherichia coli strains, Emerging Microbes & Infections, 8:1, 486-502, DOI: 10.1080/22221751.2019.1595985
NO This study was supported by FONDECYT grant 1161161 to R. Vidal and CONICYT-PCHA/2014-63140238 fellowship to D. Montero. Work at USC-LREC was supported by Project PI16/01477 from Plan Estatal de I+D+I 2013-2016, Instituto de Salud Carlos III, Subdirección General de Evaluación y Fomento de la Investigación and FEDER, Ministerio de Economia, Industria y Competitividad, Gobierno de España and Project ED431C 2017/57 from the Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia and FEDER. Fondecyt 11150966 to Felipe Del Canto. Consejo Nacional de Ciencia y Tecnología; [Fondo Nacional de Desarrollo Científico y Tecnológico].
DS Minerva
RD 28 abr 2026