RT Journal Article
T1 Single and combined effects of regulated and emerging mycotoxins on viability and mitochondrial function of SH-SY5Y cells
A1 Pérez Fuentes, Nadia
A1 Alvariño Romero, Rebeca
A1 Alfonso Rancaño, María Amparo
A1 González Jartín, Jesús María
A1 Gegunde Mosquera, Sandra
A1 Rodríguez Vieytes, Mercedes
A1 Botana López, Luis Miguel
K1 Mycotoxin
K1 Emerging
K1 Cytotoxicity
K1 Antagonistic
K1 Apoptosis
AB Co-occurrence of emerging and regulated mycotoxins in contaminated samples has been widely documented, but studies about their combined toxicity are scarce. In this report, the regulated mycotoxins deoxynivalenol, fumonisin B1 and zearalenone, and the emerging ones enniatin A, enniatin B and beauvericin were tested in SH-SY5Y human neuroblastoma cells. Their individual and binary combined effects on cell viability and mitochondrial function were evaluated. The results with individual mycotoxins revealed that deoxynivalenol and emerging mycotoxins were the most damaging to neuronal cells, presenting IC50 values between 0.35 and 2.4 μM. Interestingly, non-regulated mycotoxins triggered apoptosis by affecting to mitochondrial membrane potential. However, when regulated and non-regulated mycotoxins were binary mixed, antagonistic effects were found in all cases. Finally, cow feed and milk extracts were analysed by UHPLC-MS/MS, detecting the presence of several mycotoxins included in this study. These extracts were tested in neuroblastoma cells, and damaging effects on cell viability were found. Although binary combinations of mycotoxins produced antagonistic effects, their mixture in natural matrixes induces greater effects than expected. Therefore, it would be interesting to explore the matrix influence on mycotoxin toxicity, and to continue studying the neurotoxic mechanism of action of emerging mycotoxins, as they could be a health hazard
PB Elsevier
SN 0278-6915
YR 2021
FD 2021
LK http://hdl.handle.net/10347/27107
UL http://hdl.handle.net/10347/27107
LA eng
NO Food and Chemical Toxicology 154 (2021) 112308
NO The research leading to these results has received funding from the following FEDER cofunded grants. From Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia, 2017 GRC GI-1682 (ED431C 2017/01). From Ministerio de Ciencia e Innovación IISCIII/PI19/001248. From European Union Interreg Alertox-Net EAPA-317-2016, Interreg Agritox EAPA-998-2018, and H2020 778069-EMERTOX
DS Minerva
RD 24 abr 2026