RT Journal Article
T1 Meibum Lipidomic Analysis in Evaporative Dry Eye Subjects
A1 García Queiruga, Jacobo
A1 Pena Verdeal, Hugo
A1 Sabucedo Villamarín, Belén
A1 Paz Tarrío, Mónica
A1 Guitián Fernández, Esteban
A1 García Resúa, Carlos
A1 Yebra-Pimentel Vilar, Eva
A1 Giráldez Fernández, María Jesús
K1 meibum
K1 tear film lipid layer
K1 eyelid margin abnormalities
K1 meibomian gland
K1 lipid layer pattern
AB Meibomian Glands (MG) are sebaceous glands responsible for the production of meibum, the main component of the Tear Film Lipid Layer (TFLL). The TFLL facilitates the spread of the tear film over the ocular surface, provides stability and reduces tear evaporation. Alterations in meibum composition lead to different ocular alterations like Meibomian Gland Dysfunction (MGD) and subsequent Evaporative Dry Eye (EDE). The aim of the present study was to investigate the composition and abundance of meibum lipids and their relationship with eyelid margin abnormalities, lipid layer patterns and MG status. The study utilizes a lipidomic approach to identify and quantify lipids in meibum samples using an Elute UHPLC system. This system considered all four dimensions (mass/charge, retention time, ion mobility and intensity) to provide the accurate identification of lipid species. Samples were categorized as healthy or low/no signs of alteration (group 1) or severe signs of alteration or EDE/MGD (group 2). The current investigation found differences in Variable Importance in Projection lipid abundance between both groups for the MGD signs studied. Changes in meibum composition occur and are related to higher scores in eyelid margin hyperaemia, eyelid margin irregularity, MG orifice plugging, MG loss and lipid layer pattern.
PB MDPI
SN 1422-0067
YR 2024
FD 2024
LK http://hdl.handle.net/10347/33687
UL http://hdl.handle.net/10347/33687
LA eng
NO Int. J. Mol. Sci. 2024, 25(9), 4782
NO Convocatoria de Axudas para o Uso das Infraestructuras de Investigación da Universidade de Santiago de Compostela, número 2021-AD039
DS Minerva
RD 23 abr 2026