RT Journal Article T1 Application of Pulsed Electric Fields for Obtaining Antioxidant Extracts from Fish Residues A1 Franco Ruiz, Daniel A1 Munekata, Paulo E. S. A1 Agregán Pérez, Rubén A1 Bermúdez Piedra, Roberto A1 López-Pedrouso, María A1 Pateiro Moure, Mirian A1 Lorenzo Rodríguez, José Manuel K1 Sea Bream K1 Sea Bass K1 By-Products K1 Antioxidant Capacity K1 Amino Acid AB Fish processing has serious economic and environmental costs in the food supply chain. It is necessary to find new ways to convert fish residue to added-value products, especially for main aquaculture species. In this study, a pulsed electric field (PEF) process for antioxidant extract production from three residues (gills, bones, and heads) of two commercial species (sea bream and sea bass) was tested. Three methods of extraction using two solvents (water and methanol) and a water extraction assisted by PEF were assessed. Chemical and mineral compositions, as well as amino acid profile of the by-products, were determined. In addition, four in vitro antioxidant methods, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH), 2,2-azinobis-(3-ethyl-benzothiazoline-6-sulphonate radical (ABTS), ferric reducing antioxidant power assay (FRAP), and oxygen radical absorbance capacity assay (ORAC), were used to evaluate the extracts. Antioxidant activity was confirmed by DPPH and ABTS and FRAP tests, obtaining the highest values for residues from the sea bream species. ORAC values were higher in methanol than in water solvent. In general, gills were the residues with the greatest antioxidant activity for the four antioxidant assays employed. For DPPH assay, the extracts of water assisted by PEF from heads, bones, and gills yielded significant increases of 35.8%, 68.6%, and 33.8% for sea bream and 60.7%, 71.8%, and 22.1% for sea bass, respectively, with respect to water extracts. Our results suggest that PEF would be an environmentally friendly and economic choice for antioxidant-extract production from low-value by-products from fish processing PB MDPI YR 2020 FD 2020 LK http://hdl.handle.net/10347/21940 UL http://hdl.handle.net/10347/21940 LA eng NO Franco, D.; Munekata, P.E.S.; Agregán, R.; Bermúdez, R.; López-Pedrouso, M.; Pateiro, M.; Lorenzo, J.M. Application of Pulsed Electric Fields for Obtaining Antioxidant Extracts from Fish Residues. Antioxidants 2020, 9, 90. NO His research received external funding by the EU Commission through the BBI-JU H2020 Project AQUABIOPRO-FIT “Aquaculture and agriculture biomass side stream proteins and bioactives for feed, fitness and health promoting nutritional supplements” (Grant Agreement no. 790956). Authors would like to acknowledge to GAIN (Axencia Galega de Innovación, Xunta de Galicia, Spain) for supporting this review (grant number IN607A2019/01). Paulo E.S. Munekata acknowledges postdoctoral fellowship support from the Ministry of Economy and Competitiveness (MINECO, Spain) “Juan de la Cierva” program (FJCI-2016-29486) DS Minerva RD 24 abr 2026