RT Journal Article
T1 Environmental proteomics as a useful methodology for early-stage detection of stress in anammox engineered systems
A1 Guzmán-Fierro, Víctor
A1 Diéguez-Seoane, Alberto
A1 Roeckel, Marlene
A1 Lema Rodicio, Juan Manuel
A1 Trueba Santiso, Alba María
K1 Anammox process
K1 Industrial effluents
K1 Nitrogen removal
K1 Proteomics
K1 Stress-monitoring
K1 Wastewater treatability
AB Anammox bacteria are widely applied worldwide for denitrification of urban wastewater. Differently, their application in the case of industrial effluents has been more limited. Those frequently present high loads of contaminants, demanding an individual evaluation of their treatability by anammox technologies. Bioreactors setting up and recovery after contaminants-derived perturbations are slow. Also, toxicity is frequently not acute but cumulative, which causes negative macroscopic effects to appear only after medium or long-term operations. All these particularities lead to relevant economic and time losses. We hypothesized that contaminants cause changes at anammox proteome level before perturbations in the engineered systems are detectable by macroscopic analyses. In this study, we explored the usefulness of short-batch tests combined with environmental proteomics for the early detection of those changes. Copper was used as a model of stressor contaminant, and anammox granules were exposed to increasing copper concentrations including previously reported IC50 values. The proteomic results revealed that specific anammox proteins involved in stress response (bacterioferritin, universal stress protein, or superoxide dismutase) were overexpressed in as short a time as 28 h at the higher copper concentrations. Consequently, EPS production was also increased, as indicated by the alginate export family protein, polysaccharide biosynthesis protein, and sulfotransferase increased expression. The described workflow can be applied to detect early-stage stress biomarkers of the negative effect of other metals, organics, or even changes in physical-chemical parameters such as pH or temperature on anammox-engineered systems. On an industrial level, it can be of great value for decision-making, especially before dealing with new effluents on facilities, deriving important economic and time savings.
SN 0048-9697
YR 2024
FD 2024
LK http://hdl.handle.net/10347/33167
UL http://hdl.handle.net/10347/33167
LA eng
NO Science of The Total Environment Volume 912, 20 February 2024, 169349
NO Marlene Roeckel and Víctor Guzmán-Fierro were supported by Agencia Nacional de Investigación y Desarrollo [1200583 (FONDECYT) and 2018-21180541]. Juan M. Lema Rodicio and Alba Trueba-Santiso belong to the Galician Competitive Research Group (GRC) ED431C-2021/37. Alba Trueba-Santiso acknowledges a Juan de la Cierva-Formación postdoctoral Grant (FJC2019-041664-I).
DS Minerva
RD 25 abr 2026