RT Journal Article
T1 Diversity of Anaplasma species and importance of mixed infections in roe deer from Spain
A1 Remesar Alonso, Susana
A1 Prieto Lago, Alberto
A1 García-Dios, David
A1 López-Lorenzo, Gonzalo
A1 Martínez Calabuig, Néstor
A1 Díaz Cao, José Manuel
A1 Panadero Fontán, Rosario
A1 López Sández, Ceferino Manuel
A1 Fernández Rodríguez, Gonzalo
A1 Díez Baños, Pablo
A1 Morrondo Pelayo, María Patrocinio
A1 Díaz Fernández, Pablo
K1 Anaplasma bovis
K1 Anaplasma capra
K1 Anaplasma ovis
K1 Anaplasma phagocytophilum
K1 Anaplasma platys
K1 Roe deer
AB Although wildlife can act as reservoirs of some Anaplasma species, studies on the presence and distribution of Anaplasma spp. in wild cervids are mainly limited and focused on zoonotic species. In order to identify the Anaplasma species in roe deer from Spain and to detect co-infections, 224 spleen samples were tested for Anaplasma spp. using a commercial qPCR; positive samples were further characterized using generic 16S rRNA primers and species-specific primers targeting the msp2 and groEL genes. Anaplasma DNA was detected in the 50.9% of samples, and four Anaplasma species were identified. Anaplasma phagocytophilum (43.8%) was predominant, followed by Anaplasma bovis (13.8%), Anaplasma capra (5.8%) and Anaplasma ovis (2.2%). In addition, strains similar to Anaplasma platys were found in nine animals. Most positive roe deer (71.9%) were infected with a single Anaplasma species, whereas co-infections with two (19.3%) or three (8.8%) Anaplasma species were also found. This study confirms the widespread occurrence of Anaplasma spp. in roe deer from Spain, being the first report of A. platys-like strains and A. capra in this cervid; it is also the first report of A. capra in Spain. The detection of Anaplasma species pathogenic for humans and/or domestic animals in roe deer suggests that this cervid may play a role in the sylvatic cycle of these bacteria contributing to the appearance of clinical anaplasmosis cases. In addition, co-infections are common in roe deer revealing that Anaplasma species specific PCR assays are essential for a reliable identification as well as for determining their real prevalence
PB Wiley
YR 2021
FD 2021
LK http://hdl.handle.net/10347/29131
UL http://hdl.handle.net/10347/29131
LA eng
NO Transbound Emerg Dis. 2022;69:e374–e385. https://doi.org/10.1111/tbed.14319
NO This research was supported by a project grant awarded by the Spanish Roe Deer Association (2016-CL018; Asociación del Corzo Español (ACE), Spain), the Programme for Consolidating and Structuring Competitive Research Groups (GRC2019/04; Xunta de Galicia, Spain)
DS Minerva
RD 24 abr 2026