RT Journal Article
T1 Lactoferrin-loaded nanostructured lipid carriers (NLCs) as a new formulation for optimized ocular drug delivery
A1 Varela Fernández, Rubén
A1 García Otero, Xurxo
A1 Díaz Tomé, Victoria
A1 Regueiro, Uxía 
A1 López López, Maite
A1 González Barcia, Miguel
A1 Lema Gesto, María Isabel
A1 Otero Espinar, Francisco Javier
K1 Nanostructured lipid carriers (NLC)
K1 Double emulsification-solvent evaporation method
K1 Lactoferrin
K1 Ocular drug delivery
K1 Topical ophthalmic administration
K1 Keratoconus
AB Nanostructured lipid carriers (NLC) are novel lipidic nanosystems that provide significant improvements in terms of high drug loading capacity and controlled drug release. The purpose of the present work was based on the design, development, and physicochemical characterization of lactoferrin-loaded NLCs as a new therapeutic alternative for the keratoconus treatment. Lactoferrin-loaded NLCs were successfully prepared by a double emulsion/solvent evaporation method. The resultant NLC were assessed in terms of particle size, size distribution, surface charge, morphology, encapsulation efficiency (EE), loading capacity (LC), stability, cytotoxicity, in vitro release, and ocular surface retention. Resulting data showed a size of 119.45 ± 11.44 nm, a 0.151 ± 0.045 PDI value and a surface charge of −17.50 ± 2.53 mV. Besides, high EE and LC values were obtained (up to 75%). The in vitro release study demonstrated a lactoferrin controlled release pattern. NLCs were also stable, non-toxic and show mucoadhesive properties. Thus, a consistent preclinical base was obtained, where NLC may be considered as a potential controlled release novel drug delivery system of lactoferrin for the keratoconus treatment
PB Elsevier
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29014
UL http://hdl.handle.net/10347/29014
LA eng
NO European Journal of Pharmaceutics and Biopharmaceutics 172 (2022) 144-156
NO RVF and XGO acknowledge the financial support of the FIDIS (Health Research Institute of Santiago de Compostela Foundation). SEM and TEM analysis were feasible thanks to the Electronic and Confocal Microscopy Unit of the University of Santiago de Compostela (USC) (CACTUS, Spain). The work was partially supported by the Spanish Ministry of Science, Innovation and Universities (RTI2018-099597-B- 100)
DS Minerva
RD 29 abr 2026