RT Journal Article
T1 Vancomycin-loaded methylcellulose aerogel scaffolds for advanced bone tissue engineering
A1 Iglesias-Mejuto, Ana
A1 Magariños Ferro, Beatriz
A1 Ferreira Gonçalves, Tânia
A1 Starbird Pérez, Ricardo
A1 Álvarez Lorenzo, Carmen
A1 Reis, Catarina Pinto
A1 Ardao Palacios, Inés
A1 García González, Carlos A.
K1 3D-printing
K1 Aerogels
K1 Supercritical sterilization
K1 Vancomycin
AB Scaffolds grafting combined with local delivery of antibiotics at the injury site may promote bone regeneration along with prevention of infections. In this work, a processing strategy combining the 3D-printing of polysaccharide-based inks with supercritical (sc)CO2 technology was employed to manufacture drug-loaded, nanostructured, and personalized-to-patient aerogels for the first time. Methylcellulose (MC) was employed as graft matrix endowed with nanohydroxyapatite (nHA) to confer bioactivity as required in bone tissue engineering (BTE). MC-nHA aerogels were obtained through the 3D-printing of hydrogel-based scaffolds followed by scCO2 drying. Aerogels were loaded with vancomycin (VAN), an antibiotic employed in the management of bone infections. Textural properties and printing fidelity of scaffolds were studied as well as VAN release, long-term bioactivity, and pre-osteoblasts mineralization. In vitro cell studies and in vivo Artemia salina tests were carried out to evaluate the potential toxicity of the antibiotic-loaded aerogels. Aerogels efficacy in inhibiting bacterial growth was assessed by antimicrobial tests with Staphylococcus aureus. Textural stability of the aerogels after 7 months of storage was also evaluated. Obtained results showed that the scaffolds promoted the intended two-in-one effect (bone repair and infection management simultaneously) in a personalized way, regulating formulation design, drug dose, and porosity
PB Elsevier
SN 0144-8617
YR 2024
FD 2024
LK http://hdl.handle.net/10347/32975
UL http://hdl.handle.net/10347/32975
LA eng
NO Carbohydrate Polymers, Volume 324, 2024, 121536
NO This work was supported by MICINN [PID2020-120010RB-I00/AEI/10.13039/501100011033; PDC2022-133526-I00/AEI/10.13039/501100011033], Xunta de Galicia [ED431C 2020/17], Agencia Estatal de Investigación [AEI] and FEDER funds. Work carried out in the framework of the COST Action CA18125 “Advanced Engineering and Research of aeroGels for Environment and Life Sciences” (AERoGELS) and funded by the European Commission. A.I.-M. acknowledges to Xunta de Galicia [ED481A-2020/104] and T.F.-G. to Fundação para a Ciência e a Tecnologia (FCT) [SFRH/BD/147306/2019] for their predoctoral research fellowships
DS Minerva
RD 28 abr 2026