RT Journal Article
T1 A common garden experiment supports a genetic component underlying the increased resilience of common cockle (Cerastoderma edule) to the parasite Marteilia cochillia
A1 Villalba García, Antonio
A1 Coimbra, Maria Raquel Moura
A1 Pampín Iglesias, Marina
A1 Iglesias Estepa, david
A1 Costas, Damián
A1 Mariño, Carlos
A1 Blanco Hortas, Andrés
A1 Vera Rodríguez, Manuel
A1 Domínguez, Marta
A1 Cacabelos, Eva
A1 Abella, Emilio
A1 Incera, Mónica
A1 Fernández Otero, Rosa
A1 Martínez Portela, Paulino
K1 Bivalve mollusk
K1 Candidate genes
K1 Genetic differentiation
K1 Natural selection
K1 Single nucleotide polymorphism
AB The common cockle is a valuable bivalve species inhabiting the Atlantic European coasts. The parasite Marteilia cochillia has devastated cockle beds in the southern Galician (NW Spain) rias since 2012. Previous data suggested that cockles from Ría de Arousa acquired some resilience to this parasite through natural selection after consecutive annual marteiliosis outbreaks and candidate markers associated with marteiliosis resilience were identified using population genomics and transcriptomics approaches. Here, a common garden experiment was performed using a naïve stock (from Ría de Muros-Noia) and an affected stock (from Ría de Arousa) to test this hypothesis. Breeders from both stocks were used to produce seed cohorts at hatchery, which were pre-grown in a raft (outdoor nursery stage) and deployed in two shellfish beds affected by marteiliosis in Ría de Arousa (growing-out stage). In both beds, the naïve stock showed high marteiliosis prevalence and was fully depleted in a short period, while the affected stock barely showed evidence of marteiliosis. A set of 45 SNPs putatively associated with marteiliosis resilience were fitted for MassARRAY genotyping to check their role in the differential resilience detected between both stocks. Though no significant differentiation was found between the naïve and the affected stocks with neutral markers, 28 SNPs showed significant divergence between them, suggesting that these SNPs were involved in directional selection during eight generations (to the most) of marteiliosis pressure (long-term selection). Furthermore, signals of selection were also detected in the naïve stock along the marteiliosis outbreak in the growing-out stage (short-term selection) and six SNPs, all shared with the long-term evaluation, showed consistent signals of differentiation according to the infection severity. Some of these SNPs were located within immune genes pertaining to families such as proteasome, ubiquitin, tumor necrosis factor, and glutathione S-transferase. These resilience-associated markers will be useful to recover cockle production in Galicia
PB Wiley
SN 1752-4563
YR 2023
FD 2023-10-17
LK https://hdl.handle.net/10347/46201
UL https://hdl.handle.net/10347/46201
LA eng
NO Villalba, A., Coimbra, R. M., Pampín, M., Iglesias, D., Costas, D., Mariño, C., Blanco, A., Vera, M., Domínguez, M., Cacabelos, E., Abella, E., Incera, M., Otero, R. F., & Martínez, P. (2023). A common garden experiment supports a genetic component underlying the increased resilience of common cockle (Cerastoderma edule) to the parasite Marteilia cochillia. Evolutionary Applications, 16, 1789–1804. https://doi.org/10.1111/eva.13601
NO R.M. Coimbra was supported by a scholarship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 202015/2020-3) of the Brazilian Government. This research was funded by the European Union through the project COCKLES within the INTERREG-AA program (EAPA_458/2016), by the Consellería do Mar da Xunta de Galicia, through the project RESMART (CIMA 21/07). This research has been carried out under the framework of the Spain's Recovery and Resilience Plan, and more specifically under the investment line no.1 of its component number 17, where the complementary RTDI plan with the autonomous regions of Spain is foreseen, with one of those being the Complementary RTDI Plan for Marine Science which includes the Marine Science Program for Galicia
DS Minerva
RD 23 may 2026