RT Journal Article
T1 A novel Kluyveromyces marxianus strain with an inducible flocculation phenotype
A1 Vallejo Vidal, Juan Andrés
A1 Serrat, Manuel
A1 Pérez Portuondo, Irasema
A1 Sánchez Pérez, Ángeles
A1 Ageitos Martínez, José Manuel
A1 González Villa, Tomás
K1 Kluyveromyces marxianus
K1 Inducible flocculent phenotype
K1 Polygalacturonase
K1 Ethanol
AB Flocculation is a very useful phenotype for industrial yeast strains, since it facilitates cell harvest and represents aneasy way of cell immobilization in continuous fermentation processes. The present work represents the first timethat an inducible flocculation phenotype has been generated in a non flocculent strain of Kluyveromyces marxianus.This was accomplished by expressing Saccharomyces cerevisiae FLO5 gene in K. marxianus CECT 11769 strain. TheFLO 5 gene was placed under the control of an EPG promoter, not repressed by glucose and induced by anoxia.Our experimental approach successfully generated two novel K. marxianus flocculent phenotypes: one inducibleand one constitutive. The constitutive phenotype originated from deletions in the FLO5 promoter region, indicatingthe existence of putative upstream repressor site involved in oxygen regulation of the EPG1 promoter. The novelstrains here generated had a unique set of characteristics that provided an advantage, over the wild-type strain, forthe industrial co-production of ethanol and polygalacturonase.
PB Springer
SN 2191-0855
YR 2012
FD 2012
LK http://hdl.handle.net/10347/22035
UL http://hdl.handle.net/10347/22035
LA eng
NO Vallejo, J.A., Serrat, M., Pérez-Portuondo, I. et al. A novel Kluyveromyces marxianus strain with an inducible flocculation phenotype. AMB Expr. 2, 38 (2012)
NO This work was supported by the AECID (Grant no. A/024951/09 and A/030029/10)
DS Minerva
RD 22 abr 2026