RT Journal Article
T1 Bioaccessibility and cellular transport study of silver and titanium dioxide nanoparticles from exposed seaweed and mussels using Caco- 2 cells
A1 López Mayán, Juan José
A1 Domínguez González, Raquel
A1 Barciela Alonso, María Carmen
A1 Peña Vázquez, Elena María
A1 Moreda Piñeiro, Antonio
A1 Taboada Antelo, Pablo
A1 Bermejo Barrera, Pilar
K1 Bioaccessibility
K1 Caco 2 cells
K1 Cellular transport
K1 Single-particle-ICP-MS
K1 Single-cell-ICP-MS
AB Bioavailability studies on pollution pre-concentrator organisms such as algae and mussels are necessary to ensure food safety, particularly in the case of nanomaterials whose industrial applications have increased in recent years. Thus, the bioaccessibility and the bioavailability of total Ag and Ti and AgNPs and TiO2NPs from raw and cooked seaweed (Palmaria palmata and Ulva sp.) and cooked mussels (Mytilus edulis) exposed to 1.0 mg L−1 of 15 nm PVP-AgNPs, 1.0 mg L−1 of 25 nm citrate-TiO2NPs, or 1.0 mg L−1 5 nm citrate-TiO2NPs, for 28 days, were evaluated. Samples were subjected to an in vitro process simulating human gastrointestinal digestion while cell transport from the intestinal lumen to the bloodstream was mimicked using Caco-2 cell cultures. Total Ag and Ti in the digest samples, the bioaccessible fractions, the apical, and basolateral fractions were determined by ICP-MS, while AgNPs and TiO2NPs were determined by single-particle-ICP-MS (SP-ICP-MS). Finally, the presence of nanoparticles in the Caco-2 cells was verified by single-cell-ICP-MS (SC-ICP-MS). AgNP bioaccessibility ratios were between 22% and 97% for seaweed and 18% for mussels, while for TiO2NPs were between 17% and 81% (seaweed) and 76% and 100% (mussels). Nanoparticle cellular transports were in all cases less than 1%. However, the mass percentages of Ag as NPs and Ti as NPs in the Caco-2 cells for raw and cooked seaweed were 9% and 7% and 20% and 6%, respectively. These results confirm a small transport of the nanoparticles through the Caco-2 cells under the proposed experimental conditions.
PB Springer
SN 0026-3672
YR 2025
FD 2025
LK https://hdl.handle.net/10347/41156
UL https://hdl.handle.net/10347/41156
LA eng
NO López-Mayán, J. J., Domínguez-González, R., Barciela-Alonso, M. C., Peña-Vázquez, E., Moreda-Piñeiro, A., Taboada-Antelo, P.,  Bermejo-Barrera, P. (2025). Bioaccessibility and cellular transport study of silver and titanium dioxide nanoparticles from exposed seaweed and mussels using Caco-2 cells. "Microchimica Acta", vol. 192, nº 216
NO Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. The authors wish to thank the financial support of Ministerio de Economía y Competitividad (project INNOVANANO, reference RT2018-099222-B-100), European Union (INTERREG Atlantic Area, project NANOCULTURE, reference EAPA590/2018), and Xunta de Galicia (Grupo de Referencia Competitiva, grant number ED431C_2022/29).
DS Minerva
RD 22 abr 2026