RT Journal Article
T1 Quorum quenching in cultivable bacteria from dense marine coastal microbial communities
A1 Romero Bernárdez, Manuel
A1 Martín-Cuadrado, Ana Belén
A1 Roca-Rivada, Arturo
A1 Cabello, Ana María
A1 Otero, Ana
AB Acylhomoserine lactone (AHLs)-mediated quorum-sensing (QS) processes seem to be common in the marine environment and among marine pathogenic bacteria, but no data are available on the prevalence of bacteria capable of interfering with QS in the sea, a process that has been generally termed ‘quorum quenching’ (QQ). One hundred and sixty-six strains isolated from different marine dense microbial communities were screened for their ability to interfere with AHL activity. Twenty-four strains (14.4%) were able to eliminate or significantly reduce N-hexanoyl-l-homoserine lactone activity as detected by the biosensor strain Chromobacterium violaceum CV026, a much higher percentage than that reported for soil isolates, which reinforces the ecological role of QS and QQ in the marine environment. Among these, 15 strains were also able to inhibit N-decanoyl-l-homoserine lactone activity and all of them were confirmed to enzymatically inactivate the AHL signals by HPLC-MS. Active isolates belonged to nine different genera of prevalently or exclusively marine origin, including members of the Alpha- and Gammaproteobacteria (8), Actinobacteria (2), Firmicutes (4) and Bacteroidetes (1). Whether the high frequency and diversity of cultivable bacteria with QQ activity found in near-shore marine isolates reflects their prevalence among pelagic marine bacterial communities deserves further investigation in order to understand the ecological importance of AHL-mediated QS and QQ processes in the marine environment.
PB Blackwell Publishing
YR 2011
FD 2011
LK https://hdl.handle.net/10347/38216
UL https://hdl.handle.net/10347/38216
LA eng
NO Manuel Romero, Ana-Belen Martin-Cuadrado, Arturo Roca-Rivada, Ana María Cabello, Ana Otero, Quorum quenching in cultivable bacteria from dense marine coastal microbial communities, FEMS Microbiology Ecology, Volume 75, Issue 2, February 2011, Pages 205–217, https://doi.org/10.1111/j.1574-6941.2010.01011.x
NO This work was financed by a grant from Consellería de Innovación e Industria, Xunta de Galicia PGIDIT06PXIB200045PR. M.R. was supported by a FPU fellowship from the Spanish Ministry of Science and Education and a predoctoral fellowship from Diputación de A Coruña. A.-B.M.-C. was supported by a Juan de la Cierva scholarship, from the Spanish Ministerio de Ciencia e Innovación.
DS Minerva
RD 25 abr 2026