RT Journal Article
T1 Influence of adjuvant and antigen dose on protection induced by an inactivated whole vaccine against Neospora caninum infection in mice
A1 Rojo Montejo, Silvia
A1 Collantes Fernández, Esther
A1 Regidor Cerrillo, Javier
A1 Rodríguez Bertos, Antonio
A1 Prenafeta, Antoni
A1 Gómez Bautista, Mercedes
A1 Ortega Mora, Luis Miguel
K1 Neospora caninum
K1 Inactivated vaccine
K1 Mice
K1 Adjuvant
AB In this study, the protection afforded by a Neospora caninum inactivated vaccine formulated with three different adjuvants (water-in-oil emulsion, aluminum hydroxide with CpG oligodeoxynucleotides and aluminum hydroxide with ginseng extract) and three different parasite doses (105, 5 × 105 or 106 inactivated whole tachyzoites) was evaluated using a mouse model. Mice were immunized subcutaneously twice at three-week intervals with inactivated Nc-Spain 1H tachyzoites and challenged by intraperitoneal inoculation with 106 live Nc-1 tachyzoites. The efficacy of the  immunization was evaluated in non-pregnant BALB/c mice on days 1 and 5 (acute infection phase) and days 14 and 30 (chronic infection phase) post-challenge. The results showed the ability of water-in-oil emulsion combined with inactivated 5 × 105 tachyzoites to induce protection against neosporosis during the chronic stage, limiting parasite multiplication in the brain. Aluminum hydroxide-ginseng extract and inactivated tachyzoites reduced the number of parasites circulating in the blood during acute phase but failed to limit the establishment of chronic infection. On the other hand, a dose-effect was observed in groups vaccinated with aluminum hydroxide-ginseng extract in which the lesion severity increased as the inactivated tachyzoite dose. This study demonstrates that efficacy can significantly vary depending on the adjuvant, the dose of antigen and the phase of N. caninum infection in which the vaccine is tested.
PB Elsevier
SN 0304-4017
YR 2011
FD 2011
LK https://hdl.handle.net/10347/39488
UL https://hdl.handle.net/10347/39488
LA eng
NO Silvia Rojo-Montejo, Esther Collantes-Fernández, Javier Regidor-Cerrillo, Antonio Rodríguez-Bertos, Antoni Prenafeta, Mercedes Gomez-Bautista, Luis M. Ortega-Mora, Influence of adjuvant and antigen dose on protection induced by an inactivated whole vaccine against Neospora caninum infection in mice, Veterinary Parasitology, Volume 175, Issues 3–4, 2011, Pages 220-229, ISSN 0304-4017, https://doi.org/10.1016/j.vetpar.2010.10.028.
NO Ministerio de Educación y Ciencia
NO Laboratorios HIPRA SA
DS Minerva
RD 25 abr 2026