RT Journal Article
T1 Supramolecular association studied by Fluorescence correlation spectroscopy
A1 Novo, Mercedes
A1 Al-Soufi, Wajih
K1 Fluorescence Correlation Spectroscopy
K1 Supramolecular association
K1 Slow exchange
K1 Fast exchange
K1 Binding constant
K1 Association dynamics
AB A comprehensive description of a supramolecular system involves a full understanding of its thermodynamic and dynamic properties, as well as detailed knowledge of its structure. Fluorescence Correlation Spectroscopy (FCS) constitutes a powerful technique to acquire this information. Fluorescence correlation curves show a characteristic diffusion term that is related to the binding equilibrium constant or other thermodynamic properties of the supramolecular system. The association and dissociation rate constants of the binding process can be determined in FCS when the relaxation time of the binding is faster than the observation time—a regime called fast-exchange dynamics - in opposition to the slow-exchange regime. In all cases, structural information can be inferred from the diffusional properties of the supramolecular complexes. A short overview of the use of FCS for the study of supramolecular systems is given with examples which belong to the fast and slow regime.
PB Frontiers Media S.A.
SN 2296-2646
YR 2022
FD 2022-10-31
LK https://hdl.handle.net/10347/39050
UL https://hdl.handle.net/10347/39050
LA eng
NO Novo M and Al-Soufi W (2022), Supramolecular association studied by Fluorescence correlation spectroscopy. Front. Chem. 10:1042658. doi: 10.3389/fchem.2022.1042658
NO We acknowledge funding by the Spanish Government (Ministerio de Ciencia e Innovación, grant PID 2020-120378RB-I00) and the Galician Government (Xunta de Galicia, grant ED431B 2019/18).
DS Minerva
RD 30 abr 2026