RT Journal Article
T1 Protocol for cryopreservation of the turbot parasite Philasterides dicentrarchi (Ciliophora, Scuticociliatia)
A1 Folgueira López, Iria
A1 Felipe González, Ana Paula de
A1 Sueiro Benavides, Rosa Ana
A1 Lamas Fernández, Jesús
A1 Leiro Vidal, José Manuel
K1 Philasterides dicentrarchi
K1 Miamiensis avidus
K1 Freezing
K1 Cryoprotectants
K1 Cryopreservation
K1 Optimization
AB Philasterides dicentrarchi is a free-living marine ciliate that can become an endoparasite that causes a severe disease called scuticociliatosis in cultured fish. Long-term maintenance of this scuticociliate in the laboratory is currently only possible by subculture, with periodic passage in fish to maintain the virulence of the isolates. In this study, we developed and optimized a cryopreservation protocol similar to that used for the long-term storage of scuticociliates of the genus Miamiensis. The cryogenic medium comprised ATCC medium 1651 and a combination of 11% dimethylsulfoxide and 5% glycerol. We have verified that the most important factor ensuring the efficiency of the cryopreservation procedure is the growth phase of the culture, and that ciliates should be cryopreserved at the stationary phase (around the sixth day of culture). The cryopreservation protocol described here can be used for all strains of P. dicentrarchi as well as commercial strains of Miamiensis and enables the virulence of the strains to be maintained. Finally, this cryopreservation protocol has been shown to be more effective than others routinely applied to scuticociliates, yielding a higher survival rate with a lower initial concentration of ciliates. The results obtained indicate that the cropreservation protocol enables the long-term storage of scuticociliate parasites while maintaining the virulence of the isolates. The protocol is therefore suitable for use in vaccine production and related studies
PB Elsevier
SN 0011-2240
YR 2018
FD 2018-02
LK http://hdl.handle.net/10347/18172
UL http://hdl.handle.net/10347/18172
LA eng
NO Folgueira I, de Felipe AP, Sueiro RA, Lamas J, Leiro J. (2017). Protocol for cryopreservation of the turbot parasite Philasterides dicentrarchi (Ciliophora, Scuticociliatia). Cryobiology 80:77-83. doi: 10.1016/j.cryobiol.2017.11.010
NO This is the accepted manuscript of the following article: Folgueira, I., de Felipe, A.P., Sueiro, R.A., Lamas, J. & Leiro, J. (2018). Protocol for cryopreservation of the turbot parasite Philasterides dicentrarchi (Ciliophora, Scuticociliatia). Cryobiology, 80, 77-83. doi:10.1016/j.cryobiol.2017.11.010
NO This work was financially supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 634429 (PARAFISHCONTROL),by the Ministerio de Economía y Competitividad  (Spain) under grant agreement AGL2014-57125-R and by grant GPC2014/069 from the Xunta de Galicia (Spain)
DS Minerva
RD 25 abr 2026