RT Journal Article
T1 Tracking bacterial virulence: global modulators as indicators
A1 Prieto, Alejandro
A1 Urcola, Imanol
A1 Blanco Álvarez, Jorge
A1 Dahbi Zbiti, Ghizlane
A1 Muniesa, Maite
A1 Quirós, Pablo
A1 Falgenhauer, Linda
A1 Chakraborty, Trinad
A1 Hüttener Queiroz, Mário
A1 Juárez, Antonio
K1 Clinical microbiology
K1 Microbial genetics
AB The genomes of Gram-negative bacteria encode paralogues and/or orthologues of global modulators. The nucleoid-associated H-NS and Hha proteins are an example: several enterobacteria such as Escherichia coli or Salmonella harbor H-NS, Hha and their corresponding paralogues, StpA and YdgT proteins, respectively. Remarkably, the genome of the pathogenic enteroaggregative E. coli strain 042 encodes, in addition to the hha and ydgT genes, two additional hha paralogues, hha2 and hha3. We show in this report that there exists a strong correlation between the presence of these paralogues and the virulence phenotype of several E. coli strains. hha2 and hha3 predominate in some groups of intestinal pathogenic E. coli strains (enteroaggregative and shiga toxin-producing isolates), as well as in the widely distributed extraintestinal ST131 isolates. Because of the relationship between the presence of hha2/hha3 and some virulence factors, we have been able to provide evidence for Hha2/Hha3 modulating the expression of the antigen 43 pathogenic determinants. We show that tracking global modulators or their paralogues/orthologues can be a new strategy to identify bacterial pathogenic clones and propose PCR amplification of hha2 and hha3 as a virulence indicator in environmental and clinical E. coli isolates
PB Nature Publishing Group
YR 2016
FD 2016-05-12
LK http://hdl.handle.net/10347/16259
UL http://hdl.handle.net/10347/16259
LA eng
NO Prieto, A. et al. Tracking bacterial virulence: global modulators as indicators. Sci. Rep. 6, 25973; doi: 10.1038/srep25973 (2016)
NO The authors acknowledge funding from the Spanish MICINN-FEDER (BFU2010-21836-C02-01) and Mineco (BIO2013-49148-C2-1-R and BIO2015-69085-REDC). Work in the LREC-USC-laboratory was financed by the grant CN2012/303 from Consellería de Cultura, Educación e Ordenación Universitaria (Xunta de Galicia) and The European Regional Development Fund (ERDF)
DS Minerva
RD 28 abr 2026