RT Journal Article
T1 Identification of Radial Glia Progenitors in the Developing and Adult Retina of Sharks
A1 Sánchez Farías, Nuria
A1 Candal Suárez, Eva María
K1 Müller glia
K1 Fishes
K1 Adult neurogenesis
K1 Development
K1 GFAP
K1 DCX
AB Neural stem cells give rise to transient progenitors termed neuroepithelial cells (NECs) and radial glial cells (RGCs). RGCs represent the major source of neurons, glia and adult stem cells in several regions of the central nervous system (CNS). RGCs are mostly transient in mammals, but they are widely maintained in the adult CNS of fishes, where they continue to be morphologically similar to RGCs in the mammalian brain and fulfill similar roles as progenitors and guide for migrating neurons. The retina of fishes offers an exceptional model to approach the study of adult neurogenesis because of the presence of constitutive proliferation from the ciliary marginal zone (CMZ), containing NECs, and from adult glial cells with radial morphology (the Müller glia). However, the cellular hierarchies and precise contribution of different types of progenitors to adult neurogenesis remain unsolved. We have analyzed the transition from NECs to RGCs and RGC differentiation in the retina of the cartilaginous fish Scyliorhinus canicula, which offers a particularly good spatial and temporal frame to investigate this process. We have characterized progenitor and adult RGCs by immunohistochemical detection of glial markers as glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS). We have compared the emergence and localization of glial markers with that of proliferating cell nuclear antigen (PCNA, a proliferation maker) and Doublecortin (DCX, which increases at early stages of neuronal differentiation). During retinal development, GFAP-immunoreactive NECs located in the most peripheral CMZ (CMZp) codistribute with DCX-immunonegative cells. GFAP-immunoreactive RGCs and Müller cells are located in successive more central parts of the retina and codistribute with DCX- and DCX/GS-immunoreactive cells, respectively. The same types of progenitors are found in juveniles, suggesting that the contribution of the CMZ to adult neurogenesis implies a transition through the radial glia (RG) state
PB Frontiers Media
YR 2016
FD 2016-06-20
LK http://hdl.handle.net/10347/16008
UL http://hdl.handle.net/10347/16008
LA eng
NO Sánchez-Farías N and Candal E (2016) Identification of Radial Glia Progenitors in the Developing and Adult Retina of Sharks. Front. Neuroanat. 10:65. doi: 10.3389/fnana.2016.00065
NO This work was supported by the Spanish Dirección General de Investigación Científica y Técnica-FEDER (BFU2010-15816 and BFU2014-58631-P), the Xunta de Galicia (10PXIB200051PR, IN845B-2010/159 and CN 2012/237)
DS Minerva
RD 27 abr 2026